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Enzymatic pretreatment assisted curcumin extraction technology

A technology of auxiliary extraction and curcumin, applied in the separation/purification of carbonyl compounds, organic chemistry and other directions, can solve the problems of curcumin extract that is not easy to dry, destroy curcumin activity, accelerate curcumin dissolution, etc., and achieve easy control of reaction conditions. , The effect of improving clarity and stable product quality

Inactive Publication Date: 2015-11-18
BAOTOU MEDICAL COLLEGE OF INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to increase the extraction efficiency and accelerate the stripping of curcumin, the organic solvent extraction usually needs to increase the extraction temperature, which will destroy the activity of curcumin to a certain extent, and the obtained curcumin extract is not easy to dry, and the purity of curcumin is also low. will be affected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0033] A kind of technology that enzymatic pretreatment assists to extract curcumin, it comprises the steps:

[0034] Step 1: Take 1 part of turmeric, crush it and sieve it to 60 mesh, then add 3 parts of tap water and stir evenly, seal the water bath at 60°C for 60 minutes, stir twice during the water bath, then lower the temperature and keep it constant to 52°C to obtain turmeric slurry;

[0035] Step 2: Add a compound enzyme composed of 8 U / g turmeric cellulase, 60 U / g turmeric amylase and 5 U / g turmeric papain to the turmeric slurry, and react for 2 hours at pH 3.8 to obtain the enzyme Solution;

[0036] Step 3: Take the enzymolysis solution described in step 2, add 7 times its volume multiple of ethanol solution, and homogenize for 1 min under the condition of 8000r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 65%;

[0037] Step 4: After adding chitosan of 0.3g / L in the extract and stirring for 30min, filter, and the obtained clear...

example 2

[0039] A kind of technology that enzymatic pretreatment assists to extract curcumin, it comprises the steps:

[0040] Step 1: Take 1 part of turmeric, crush it and sieve it to 80 mesh, then add 6 parts of tap water and stir evenly, seal the water bath at 70°C for 60 minutes, stir 3 times during the water bath process, then lower the temperature and keep it constant to 58°C to obtain turmeric slurry;

[0041] Step 2: Add a compound enzyme composed of 12 U / g turmeric cellulase, 90 U / g turmeric amylase and 10 U / g turmeric papain to the turmeric slurry, and react for 4 hours at pH 4.5 to obtain the enzyme Solution;

[0042] Step 3: Take the enzymolysis solution described in step 2, add 9 times its volume multiple of ethanol solution, and homogenize it for 2 minutes under the condition of 9000r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 70%;

[0043]Step 4: After adding 0.5g / L sodium polyacrylate in the extract and stirring for 60min, filt...

example 3

[0045] A kind of technology that enzymatic pretreatment assists to extract curcumin, it comprises the steps:

[0046] Step 1: Take 1 part of turmeric, crush it and sieve it to 70 mesh, then add 5 parts of tap water and stir evenly, seal the water bath at 65°C for 60 minutes, stir 3 times during the water bath process, then lower the temperature and keep it constant to 55°C to obtain turmeric slurry;

[0047] Step 2: Add a compound enzyme composed of 10 U / g turmeric cellulase, 80 U / g turmeric amylase and 7 U / g turmeric papain to the turmeric slurry, and react for 3 hours at pH 4.0 to obtain the enzyme Solution;

[0048] Step 3: Take the enzymolysis solution described in step 2, add 8 times its volume multiple of ethanol solution, and homogenize it for 1.5min under the condition of 8500r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 68%;

[0049] Step 4: Add 0.5 g / L polyacrylamide to the extract and stir for 50 minutes, then filter, pass t...

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Abstract

The invention provides an enzymatic pretreatment assisted curcumin extraction technology. The enzymatic pretreatment assisted curcumin extraction technology comprises steps as follows: after 1 part of curcuma powder is taken, ground, sieved, added with water and stirred uniformly, the mixture is subjected to water bath for 60 min and is cooled to the constant temperature of 52-58 DEG C, and curcuma pulp is obtained; compound enzyme comprising cellulase, amylase and papain is added to the curcuma pulp and reacts for 2-4 h, and enzymatic hydrolysate is obtained; the enzymatic hydrolysate is taken, added with an ethanol solution and homogenized for 1-2 min under the condition of 8,000-9,000 r / min, and a curcumin extracting solution is obtained; finally, a flocculant is added to the extracting solution, the mixture is stirred and filtered, a clear liquid flows through an activated carbon chromatographic column and is eluted with an alkaline solution, and a curcumin desorbed solution is obtained. The technology has the advantages of high extraction speed, low temperature, low energy consumption, high yield of the effective component curcumin, stable product quality and controllable reaction conditions, and is applicable to industrial large-scale production.

Description

technical field [0001] The invention relates to an extraction process of curcumin. More specifically, the present invention relates to a process for assisting the extraction of curcumin by enzymatic pretreatment, which can effectively improve the extraction efficiency and purity of curcumin. Background technique [0002] Curcumin is extracted from turmeric, a traditional Chinese medicine, and is the most important component of turmeric to exert its pharmacological effects. Curcumin can promote the formation of immune cells called macrophages, which can clear a type of amyloid that can form Alzheimer's-related, and has excellent preventive effects on Alzheimer's disease. In addition, curcumin also has physiological and pharmacological effects such as promoting gallbladder and protecting liver, lowering blood fat, promoting blood circulation and promoting menstrual flow, anti-HIV, anti-breast cancer, anti-prostate cancer, and anti-atherosclerosis. [0003] There are many rep...

Claims

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Application Information

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IPC IPC(8): C07C45/78C07C49/255C07C45/79
CPCC07C45/78C07C45/79C07C49/255
Inventor 齐瑞芳高洪波方欣石瑞丽马宝慧吕军陈晓东时静华徐继辉王立军
Owner BAOTOU MEDICAL COLLEGE OF INNER MONGOLIA UNIV OF SCI & TECH
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