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Crossbreeding and seedling rapid propagation method of paphiopedilum pacific shamrock

A cross-breeding and cymbidium-like technology, applied in the field of plant tissue culture, can solve the problems of low seed germination rate and slow reproduction speed, and achieve the effects of high application value, good seedling quality, and shortening breeding and production cycles.

Inactive Publication Date: 2015-11-18
FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the defects of the prior art, the object of the present invention is to provide a method for the hybridization and breeding of the meat-patty Paphiopedilum and its rapid propagation of seedlings, including the cultivation management measures after the seedlings are transplanted, and solve the problem of Due to the problem of low seed germination rate and slow propagation speed, it provides a specific operation method for the selection and breeding of new varieties of Paphiopedilum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for crossbreeding Paphiopedilum and its rapid propagation of seedlings comprising the following steps:

[0032] (1) Artificial pollination: choose Paphiopedilum hybrid as the female parent, self-cross, hybridize between different strains within the same species or hybridize within different species, artificially pollinate 4 days after the flowers open, and grow after pollination get a capsule;

[0033] (2) selection of seed maturity: select the fully expanded capsule of growth 300d as seed;

[0034] (3) Disinfection and sowing of fruit pods: After cutting the fruit pods, wash them with tap water, rinse them with neutral detergent, wipe them with alcohol, rinse them with running water for 15 minutes, transfer them to a super-clean workbench, and disinfect them with 75% alcohol for 30 seconds , soak in 1% sodium hypochlorite solution for 12 minutes, then rinse with sterile water for 6 times, cut the fruit pods and spread the seeds evenly on the surface of the ge...

Embodiment 2

[0040] A method for crossbreeding Paphiopedilum and its rapid propagation of seedlings comprising the following steps:

[0041] (1) Artificial pollination: choose Paphiopedilum hybrid as the female parent, self-cross, hybridize between different lines within the same species or hybridize within different species, artificially pollinate 15 days after the flowers open, and grow after pollination get a capsule;

[0042] (2) selection of seed maturity: select the fully expanded capsule of growth 360d as seed;

[0043] (3) Disinfection and sowing of fruit pods: After cutting the fruit pods, wash them with tap water, rinse them with neutral detergent, wipe them with alcohol, rinse them with running water for 15 minutes, transfer them to a super-clean workbench, and disinfect them with 75% alcohol for 30 seconds , soak in 1% sodium hypochlorite solution for 12 minutes, then rinse with sterile water for 6 times, cut the fruit pods and spread the seeds evenly on the surface of the ger...

Embodiment 3

[0049] A method for crossbreeding Paphiopedilum and its rapid propagation of seedlings comprising the following steps:

[0050] (1) Artificial pollination: choose Paphiopedilum hybrid as the female parent, self-cross, hybridize between different lines within the same species or hybridize within different species, perform artificial pollination 10 days after the flowers open, and grow after pollination get a capsule;

[0051] (2) selection of seed maturity: select the fully expanded capsule of growth 330d as seed;

[0052] (3) Disinfection and sowing of fruit pods: After cutting the fruit pods, wash them with tap water, rinse them with neutral detergent, wipe them with alcohol, rinse them with running water for 15 minutes, transfer them to a super-clean workbench, and disinfect them with 75% alcohol for 30 seconds , soak in 1% sodium hypochlorite solution for 12 minutes, then rinse with sterile water for 6 times, cut the fruit pods and spread the seeds evenly on the surface of...

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PUM

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Abstract

The invention discloses a crossbreeding and seedling rapid propagation method of paphiopedilum pacific shamrock. The method comprises the following steps: carrying out artificial pollination, selecting the ripeness of the seeds, enabling the seeds to germinate to form protocorms, and enabling protocorms to differentiate into plantlets, carrying out strong seedling culture, acclimatization and transplantation, and carrying out cultivation management on the transplanted seedlings. The method has the advantages of high hybridization setting percentage, high germination rate of hybrid seeds, low browning rate of protocorms, rapidness in seedling forming, good quality of seedlings, strong operability and high application value, the conventional protocorm subculture proliferation stage is eliminated, the breeding and production cycles are effectively shortened, the bud mutation probability and the production cost are reduced, and important significance is provided for enriching the varieties of paphiopedilum, and improving the quality of paphiopedilum.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for crossbreeding of Paphiopedilum patties and rapid propagation of seedlings thereof, including the cultivation and management technology after the seedlings come out of the bottle. Background technique [0002] Paphiopedilum hybrid is a hybrid species of the genus Paphiopedilum in the family Orchidaceae. Its flower shape is peculiar and large, and the flower color is gorgeous. It is a relatively popular Paphiopedilum cultivar in the market. [0003] Compared with the native species, the hybrid species of Paphiopedilum have more beautiful flower shapes, richer and brighter colors, and stronger resistance to stress. In the international market, hybrid flowers with Paphiopedilum as parent are deeply loved by people, and have become a major pillar of the world's orchid industry and have achieved huge economic benefits. However, the cross breeding of Paphioped...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
Inventor 李秀玲卜朝阳黄凤玲黄昌艳邓杰玲李春牛卢家仕关世凯屈婷婷
Owner FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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