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Primers, probes and kit for typing qualitative detection of hepatitis B virus (HBV)

A technology for qualitative detection of hepatitis B virus, applied in the direction of microbiological measurement/test, microbiological, microbiological-based methods, etc., can solve the problems of inability to carry out targeted treatment and inability to judge the genotype of HBV virus in patients

Inactive Publication Date: 2015-11-11
北京鑫诺美迪基因检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problem that the prior art cannot determine the genotype of the patient's HBV virus, resulting in the inability to perform targeted treatment, the present invention provides a set of primers and probes for the qualitative detection of hepatitis B virus, which can realize rapid, effective and accurate detection And type HBV virus

Method used

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  • Primers, probes and kit for typing qualitative detection of hepatitis B virus (HBV)
  • Primers, probes and kit for typing qualitative detection of hepatitis B virus (HBV)
  • Primers, probes and kit for typing qualitative detection of hepatitis B virus (HBV)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] 1. Design of primers and probes

[0073] Specific primers HBV(FX)-B-F, HBV(FX)-B-R and Taqman fluorescent probe HBV(FX)-B-FP were designed for hepatitis B virus type B. The fluorophore labeled at the 5' end of Taqman fluorescent probe JCV-P is 6-FAM, and the quencher group labeled at the 3' end is BHQ1.

[0074] Upstream primer HBV(FX)-B-F: GGGGAACACCCGTGTGTCT (SEQ ID NO: 1);

[0075] Downstream primer HBV(FX)-B-R: GTCCAGAAGAACCAACAAGAAGA (SEQ ID NO: 2);

[0076] Probe HBV(FX)-B-FP: ATTCGCAGTCCCAAATTCTCCAGTCA (SEQ ID NO: 3).

[0077] Specific primers HBV(FX)-C-F, HBV(FX)-C-R and Taqman fluorescent probe HBV(FX)-C-FP were designed for hepatitis B virus type C. The fluorophore labeled at the 5' end of Taqman fluorescent probe JCV-P is 6-FAM, and the quencher group labeled at the 3' end is BHQ1.

[0078] Upstream primer HBV(FX)-C-F: GGGGTTTTTCTTGTTGACAAG (SEQ ID NO: 4);

[0079] Downstream primer HBV(FX)-C-R: GGACAAGAGGTTGGTGAGTGAT (SEQ ID NO: 5);

[0080] Probe HBV(...

Embodiment 2

[0111] Embodiment 2 clinical sample detection

[0112] 1. Using the method of Example 1, 2 cases of hepatitis B virus type B serum samples, 5 cases of hepatitis B virus negative serum samples, 2 cases of hepatitis B virus type C serum samples, and 2 cases of hepatitis B virus type D serum samples Samples, hepatitis B virus type A serum samples, hepatitis C virus (HCV) blood samples, Epstein-Barr virus (EBV) serum samples, human cytomegalovirus (HCMV) serum samples, a total of 15 samples were tested. Test results such as figure 1 Shown:

[0113] Among them, 2 serum samples of hepatitis B virus type B had S-type amplification curves; the remaining samples included 5 serum samples negative for hepatitis B virus, 2 serum samples of hepatitis B virus type C, and 2 samples of hepatitis B virus Type D serum samples and other common clinical pathogens [including hepatitis C virus (HCV), Epstein-Barr virus (EBV), and human cytomegalovirus (HCMV)] were all negative, and there was no ...

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Abstract

The invention discloses primers, probes and a kit for typing qualitative detection of hepatitis B virus (HBV). The primers and probes for typing qualitative detection of HBV comprises B-type detection primers and probes, C-type detection primers and probes, and D-type detection primers and probes. The primers, probes and kit have the advantages of high sensitivity, favorable specificity, high reaction speed and low cost, and are suitable for large-scale clinical development. The primers, probes and kit can implement quick, effective and accurate typing qualitative detection on the HBV, thereby ensuring the timely treatment and treatment effect monitoring on the hepatitis B state.

Description

technical field [0001] The present invention relates to a kind of qualitative detection method and test kit of three kinds of genotype typing of virus, specifically, relate to a kind of qualitative detection primer, probe and reagent of three kinds of genotype typing of hepatitis B virus B, C, D box. Background technique [0002] Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and is an enveloped double-stranded DNA virus. HBV was first discovered in the serum of Australian aborigines in 1963, HBV virus particles were discovered by Dane in 1970, and HBV was classified as Hepadnaviridae in 1983. HBV is divided into 8 genotypes according to the nucleotide sequence difference of ≥8% in the whole HBV genome, or ≥4% difference in S gene sequence. [0003] HBV is present in blood and body fluids. Various types of acute and chronic hepatitis B patients and HBsAg carriers can be used as sources of infection. HBV is mainly transmitted through blood and blood products...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
CPCC12Q1/706C12Q1/6851
Inventor 刘沛朱柳王佳王林海
Owner 北京鑫诺美迪基因检测技术有限公司
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