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Application of mirna-pa compound in the preparation of diagnostic markers and therapeutic drugs for chronic pain

A compound, drug technology, applied in the field of biomedicine

Inactive Publication Date: 2018-05-15
XUZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, miRNA-Pa is a small miRNA molecule newly discovered by the applicant, and the use of miRNA-Pa as a gene for suppressing chronic pain and as a diagnostic marker for chronic pain has not yet been reported.

Method used

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  • Application of mirna-pa compound in the preparation of diagnostic markers and therapeutic drugs for chronic pain
  • Application of mirna-pa compound in the preparation of diagnostic markers and therapeutic drugs for chronic pain
  • Application of mirna-pa compound in the preparation of diagnostic markers and therapeutic drugs for chronic pain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of complete Freund's adjuvant (CFA) chronic arthritis pain model:

[0022] SPF grade male Kunming male mice of 6 weeks were selected under isoflurane anesthesia, and 10 μl of sterile complete Freund's adjuvant (CFA; Sigma Company) was injected intradermally at two points around the left hind foot metatarsal joint. This injection concentration can induce arthritis. 24 hours after injection, thermal and mechanical pain thresholds decreased and could be maintained for more than 3 weeks, and they were regarded as qualified mice for chronic pain modeling.

[0023] Pain behavior monitoring: Thermal hyperalgesia: Place the plexiglass box on a 3mm thick glass plate, and use thermal radiation stimulation according to the Hargreaves method to irradiate the mouse's sole close to the glass plate. Record the time from the beginning of irradiation to the appearance of the mouse's foot lifting, and the cut-off time is 20s. Each animal was measured 3 times, and the ave...

Embodiment 2

[0038] Example 2 miRNA-Pa treatment of chronic pain animal experiments

[0039] 1. Modeling and grouping:

[0040] SPF grade male Kunming male mice of 6 weeks were selected under isoflurane anesthesia, and 40 μl of sterile complete Freund's adjuvant was intradermally injected at two points around the left hind foot metatarsal joint. This injection concentration can induce arthritis. 24 hours after the injection, thermal and mechanical pain thresholds decreased and could be maintained for more than 3 weeks, as qualified mice for modeling; 20 mice in the miRNA-Pa intervention treatment group and 20 mice in the model control group (with phosphate buffer solution) PBS injection).

[0041] 2. Experimental method:

[0042] Intrathecal injection of miRNA-Pa intervention treatment group: After the mice were anesthetized with isoflurane, they were injected three times with miRNA-Pa-mimics analogs overexpressing miRNA-Pa (concentration 20 μM), 5 μl each time, along the spinal cord dur...

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PUM

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Abstract

The invention discloses the application of the miRNA-Pa compound in the preparation of clinical diagnostic markers for chronic pain diseases and in the preparation of medicines for treating chronic pain. The present invention screens through a large number of experiments, and the experimental results show that the expression of miRNA-Pa is down-regulated in the blood of chronic arthritis pain models and chronic pain patients. By up-regulating miRNA-Pa, the occurrence and formation of chronic pain can be significantly inhibited, and the blood Quantitative analysis of clinical samples demonstrated that miRNA‑Pa could serve as a marker of chronic pain development.

Description

technical field [0001] The invention relates to a new application of microRNA molecules, in particular to a new application of miRNA-Pa, and belongs to the technical field of biomedicine. Background technique [0002] MicroRNA (miRNA) is a newly discovered small 18-22bp single-stranded RNA molecule that regulates gene expression at the post-transcriptional level. miRNA is involved in various biological processes such as cell differentiation, apoptosis and metabolism, and the abnormality of this regulatory process can cause a variety of diseases. Recent studies have shown that miRNA, as an important regulator of gene expression regulation, plays an important regulatory role in neurophysiological and pathological processes, such as neuronal development, neuronal plasticity changes, and nervous system diseases. Chronic pain is one of the most common clinical diseases that endanger human health and reduce people's quality of life, and has become a hot issue in clinical and basi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883A61K31/7088A61P29/00
CPCA61K9/0053A61K31/7088C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 潘志强曹君利薛洲亚李国芳张铭郝凌云
Owner XUZHOU MEDICAL COLLEGE
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