Mulberry enzyme health beverage production method
A health-care beverage and mulberry enzyme technology, applied in the field of bioengineering, can solve the problems of long fermentation time, pollution, and the inability to guarantee the absence of pathogenic bacteria, and achieve the effects of reducing loss, retaining enzyme activity and maintaining stable quality.
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Embodiment 1
[0023] A method for fermenting mulberry enzymes, comprising the following steps:
[0024] (1) Select mature mulberry with fresh color and high natural enzyme content as the main raw material for fermentation.
[0025] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 30% of the volume, add 10% of the total liquid volume % of the raw honey is for the fermentation stock solution.
[0026] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0027] (4) According to the inoculation ratio of 5%, the nectar-derived bacteria and lactobacillus were respectively taken and inserted into the fermentation stock solution, and fermented for 7 days, with a pH value of 7.0 and a temperature of 25°C.
[0028] (5) Coarse filtration is carried out first...
Embodiment 2
[0030] A method for fermenting mulberry enzymes, comprising the following steps:
[0031] (1) Select mature mulberry with fresh color and high natural enzyme content as the main raw material for fermentation.
[0032] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 40% of the volume, add 15% of the total liquid volume % of the raw honey is for the fermentation stock solution.
[0033] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0034] (4) According to the inoculation ratio of 4%, the nectar-derived bacteria and lactic acid bacteria were respectively taken and inserted into the fermentation stock solution, and fermented for 10 days, with a pH value of 6.5 and a temperature of 28°C.
[0035] (5) Coarse filtration is carried o...
Embodiment 3
[0037] A method for fermenting mulberry enzymes, comprising the following steps:
[0038] (1) Select mature mulberry with fresh color and high natural enzyme content as the main raw material for fermentation.
[0039] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 50% of the volume, add 20% of the total liquid volume % of the raw honey is for the fermentation stock solution.
[0040] (3) Prepare LB medium, activate the original preserved strain, and cultivate the fermented bacterial liquid obtained for about 48 hours for later use; (4) According to the inoculation ratio of 3%, the nectar-derived bacteria, Lactobacillus casei and Lactobacillus plantarum were respectively taken and inserted The fermentation stock solution was fermented for 14 days, with a pH value of 7.0 and a temperature of 25°C.
[0041] (5) Coarse filtration is carried out fi...
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