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Alpha-amylase detection reagent and application thereof

A technology for detecting reagents and amylase, applied in the field of medical testing, can solve problems such as poor repeatability of measurement results and errors in measurement results, and achieve the effects of stable reagents, reduced errors and good repeatability

Active Publication Date: 2015-11-04
郑州金域临床检验中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the shortcomings of the prior art α-amylase detection reagents that have large errors in measurement results and poor repeatability of measurement results, the purpose of the present invention is to provide a α-amylase detection reagent and its application to solve the above problems

Method used

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  • Alpha-amylase detection reagent and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1, α-Amylase Detection Reagent

[0033] The α-amylase detection reagent includes reagent R1 and reagent R2, and the reagent R1 includes the following components:

[0034] MES buffer solution with a pH value of 5.5 40mmol / L, NH 4 Cl 80mmol / L, CaCl 2 5mmol / L, polyethylene glycol 3g / L, paraben 2g / L;

[0035] The reagent R2 comprises the following components:

[0036] MES buffer with a pH value of 5.5 40mmol / L, 2-chloro-4-nitrobenzene-maltotriose 10mmol / L, paraben 2g / L.

Embodiment 2

[0037] Embodiment 2, α-Amylase Detection Reagent

[0038] The α-amylase detection reagent includes reagent R1 and reagent R2, and the reagent R1 includes the following components:

[0039] MES buffer solution with pH value of 6.0 80mmol / L, NH 4 Cl 120mmol / L, CaCl 2 10mmol / L, polyethylene glycol 5g / L, paraben 6g / L;

[0040] The reagent R2 comprises the following components:

[0041] MES buffer with a pH value of 6.0 80mmol / L, 2-chloro-4-nitrobenzene-maltotriose 30mmol / L, paraben 5g / L.

Embodiment 3

[0042] Embodiment 3, α-Amylase Detection Reagent

[0043] The α-amylase detection reagent includes reagent R1 and reagent R2, and the reagent R1 includes the following components:

[0044] MES buffer solution with pH value of 6.5 100mmol / L, NH 4 Cl160mmol / L, CaCl 2 30mmol / L, polyethylene glycol 10g / L, paraben 8g / L;

[0045] The reagent R2 comprises the following components:

[0046] MES buffer with a pH value of 6.5 100mmol / L, 2-chloro-4-nitrobenzene-maltotriose 80mmol / L, paraben 8g / L.

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Abstract

The present invention belongs to the field of medical laboratory technology, and in particular relates to an alpha-amylase detection reagent and application thereof. The reagent comprises a reagent R1 and a reagent R2; the reagent R1 is mainly composed of an MES buffer, NH4Cl, CaCl2, a surfactant and a preservative; the R2 reagent consists of an MES buffer, a 2-chloro-4-nitrobenzene-maltotriose and a preservative. The alpha-amylase detection reagent provided by the invention does not contain NaN3, does not lead to alpha-amylase allosterism in the determination process, and can reduce the error in measurement results; at the same time, the test does not need additional tool enzyme, and has short delay time; the test results have good repeatability and stability. Therefore, the invention is conducive to the clinical application of alpha-amylase detection reagent.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to an α-amylase detection reagent and application thereof. Background technique [0002] The scientific name of α-amylase is 1,4-α-D-glucan-4-glucanohydrolase, which is an enzyme synthesized by epithelial cells with secretory function in the salivary gland and pancreas. α-Amylase is an important biochemical marker for detecting acute pancreatitis. Therefore, the determination of the concentration of α-amylase has important clinical significance. [0003] According to statistics, there are more than 200 methods for the determination of α-amylase, which are mainly divided into six categories: viscometry, iodometric method, turbidimetric method, saccharification method, dye release method and fluorescence method. With the continuous in-depth research on α-amylase assay methods, many new assay methods have emerged. At present, the most widely used method is the et...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 燕启江
Owner 郑州金域临床检验中心有限公司
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