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A method for extracting steviol glycosides from biological enzyme fermentation broth

A technology of steviol glycosides and fermented liquid, which is applied in the direction of sugar derivatives, sugar derivatives, sugar derivatives preparation, etc. It can solve the problems of not being able to meet the requirements of consumers, the difficulty of purifying high-purity products, and the restricted supply of raw materials. Achieve the effect of reducing regeneration maintenance cost, ensuring uniformity, preventing over-fermentation or

Active Publication Date: 2018-01-23
QINGDAO RUNDE BIOTECH
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Problems solved by technology

[0004] However, the stevioside industry currently extracts steviosides from stevia leaves. Compared with other synthetic sweeteners, the production and preparation of traditional steviosides have difficulties in the purification of high-purity products, high prices and restricted raw materials. Due to the unstable supply of agricultural planting and many other problems
Therefore, the production and preparation of traditional steviosides can no longer meet the requirements of consumers, and it is necessary to seek a new technical means that can solve existing problems and is superior to traditional extraction of steviosides

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  • A method for extracting steviol glycosides from biological enzyme fermentation broth
  • A method for extracting steviol glycosides from biological enzyme fermentation broth
  • A method for extracting steviol glycosides from biological enzyme fermentation broth

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Embodiment Construction

[0027]The monosaccharide bio-enzyme fermentation liquid selected in this embodiment is the fermentation liquid produced by the fermentation of Saccharomyces species. The method of the present invention for extracting high-purity steviol glycoside RA from monosaccharide fermentation liquid, firstly, pretreating the monosaccharide biological enzyme fermentation liquid:

[0028] Using saturated steam, 3502 ml of fermentation broth was heated to 95°C and maintained for 20 minutes. Use circulating cooling water to reduce the temperature of the primary clarified fermentation broth to ≤50°C. Add citric acid to adjust the pH of the monosaccharide fermentation broth to 4.0. Add 8% of the "natural organic polymer polysaccharide composite flocculant" configuration solution, and use a high-speed centrifuge with a separation factor ≥ 5000 to perform a decontamination and separation of the above-mentioned pretreated fermentation broth, with a light transmittance ≥ 80, to obtain a clarifica...

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Abstract

The invention provides a method for extracting stevioside from biological enzyme fermentation liquid. According to the method, monosaccharide biological enzyme fermentation liquid serves as raw materials, and high-purity stevioside RA is extracted. The extraction method includes monosaccharide biological enzyme fermentation liquid preprocessing and stevioside purification. Fermentation liquid preprocessing includes the steps of fermentation liquid heating, primary PH value adjusting, flocculation and sedimentation, centrifugal separation, secondary PH value adjusting, cooling and membrane filtering. Stevioside purification includes the steps of macroporous resin absorption-parsing, cation and anion exchange, activated carbon treatment, desolventizing-concentration, dewatering and drying, primary crystal, secondary crystal, dewatering and drying and finished product obtaining. The stevioside RA extracted from the monosaccharide biological enzyme fermentation liquid is high in purity, excellent in colour and luster, pure in taste and free of peculiar smell. By means of the extraction method, energy is saved, environmental protection is facilitated, and the manufacturing cost of high-end stevia sugar is lowered; meanwhile, a new raw material supply channel is opened up, the quality of raw materials of the stevioside is more stable, and the number of raw materials is more sufficient.

Description

technical field [0001] The present invention relates to the processing technology of fermented liquid, more relates to the processing technology of extracting steviol glycoside from fermented liquid, especially relates to a method for extracting high-purity steviol glycoside RA (Rebaudioside A) from monosaccharide fermented liquid. Background technique [0002] Nowadays, human beings have a general preference for sweet foods. However, since a large amount of sucrose intake can cause various adult diseases such as diabetes, obesity, and heart disease, it has become a social problem. Choosing the right sweetener plays a very important role in our daily life as well as in the food, beverage, and pharmaceutical industries. [0003] Stevia is a new type of natural sweetener extracted from the herb Stevia rebaudiana of Compositae. It not only has the characteristics of high sweetness, low calorie, non-toxic side effects, non-carcinogenic food safety, etc., but also has unique the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/256C07H1/06
CPCY02P20/10
Inventor 张永李存彪夏正一
Owner QINGDAO RUNDE BIOTECH
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