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Method for preparing AuNPs@PDA/PLGA nanocapsule

A nanocapsule and solution technology, applied in the field of ultrasound medicine, can solve the problems of HIFU focused energy interference, easy damage to surrounding normal tissues, and long time required for ablation, and achieve good in vitro contrast-enhanced ultrasound imaging capabilities, simple design methods, and enhanced curative effect Effect

Inactive Publication Date: 2015-11-04
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The application of HIFU is very extensive. However, with the deepening of its application, there are still many problems that need to be solved urgently in clinical application, such as the long time required for ablation, incomplete ablation, repeated ablation of the target area, easy to damage the surrounding normal tissues, and the existence of HIFU. Interfering factors of focused energy, etc.

Method used

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  • Method for preparing AuNPs@PDA/PLGA nanocapsule
  • Method for preparing AuNPs@PDA/PLGA nanocapsule
  • Method for preparing AuNPs@PDA/PLGA nanocapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of PLGA nanocapsules: first mix 0.1 g of PLGA material with 2 mL of dichloromethane solution, then add 0.2 mL of deionized water dropwise, then add 10 mL of 5% PVA solution at 9500-10000 r / min After emulsifying with a high-speed dispersing homogenizer for 3 min, 50 mL of 0.3% PVA solution was added to the mixture and stirred overnight at room temperature (at a speed of 750 r / min for 12 hours), centrifuged, and washed with water for 4 times to obtain a solid Mutually.

[0033] (2) Preparation of PDA / PLGA nanocapsules: 100 mg of the PLGA nanocapsule solid phase prepared above was dissolved in 50 mL of Tris aqueous solution (pH=8.5, 0.01 M) and 25 mg of dopamine hydrochloride was added. The mixture was stirred at a speed of 5 min for 5 hours, centrifuged, and washed 4 times with water to obtain a solid phase.

[0034] (3) Preparation of AuNPsPDA / PLGA nanocapsules:

[0035] Dissolve 100 mg of the PDA / PLGA nanocapsules prepared above in aqueous solution, ad...

Embodiment 2

[0037] (1) Preparation of PLGA nanocapsules: first mix 0.1 g of PLGA material with 2 mL of dichloromethane solution, then add 0.2 mL of aqueous solution dropwise, then add 10 ml of 5% PVA solution at a high speed of 9500-10000 r / min After emulsifying with a homogenizer for 3 min, add 50 mL of 0.3% PVA solution to the mixture and stir overnight at room temperature (at a speed of 750 r / min for 12 hours), centrifuge, and wash with water 4 times to obtain a solid phase.

[0038] (2) Preparation of PDA / PLGA nanocapsules: 100 mg of the PLGA nanocapsule solid phase prepared above was dissolved in 50 mL of Tris solution (pH = 8.5, 0.01M) and 50 mg of dopamine hydrochloride was added. The mixture was stirred at a speed of 5 min for 5 hours, centrifuged, and washed 4 times with water to obtain a solid phase.

[0039] (3) Preparation of AuNPsPDA / PLGA nanocapsules:

[0040]Take 100 mg of the PDA / PLGA nanocapsules prepared above, dissolve them in aqueous solution, add dropwise 44 mL of ch...

Embodiment 3

[0042] (1) Preparation of PLGA nanocapsules: first mix 0.1 g of PLGA material with 2 mL of dichloromethane solution, then add 0.2 mL of deionized water dropwise, then add 10 mL of 5% PVA solution at 9500-10000 r / min After emulsifying with a high-speed dispersing homogenizer for 3 min, 50 mL of 0.3% PVA solution was added to the mixture and stirred overnight at room temperature (the speed was 750 r / min, and the time was 12 hours), centrifuged, and washed with water 4 times to obtain the solid phase.

[0043] (2) Preparation of PDA / PLGA nanocapsules: Take 100 mg of the PLGA nanocapsule solid phase prepared above, dissolve it in 50 mL Tris solution (pH=8.0, 0.01 M) and add 100 mg of dopamine hydrochloride. The mixture was stirred at a speed of 6 min for 6 hours, centrifuged, and washed 4 times with water to obtain a solid phase.

[0044] (3) Preparation of AuNPsPDA / PLGA nanocapsules:

[0045] Take 100 mg of the PDA / PLGA nanocapsules prepared above, dissolve them in aqueous solu...

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Abstract

The invention discloses a method for preparing an AuNPs@PDA / PLGA nanocapsule and belongs to the field of ultrasonic medicine. According to the method, a PDA / PLGA nanocapsule is formed firstly through auto polymerization of dopamine in an alkalescence aqueous environment, then a chloroauric acid solution is added, and AuCl4-ions are adsorbed by PDA molecules onto the surface of the PDA / PLGA nanocapsule and reduced to nanogold particles to form the AuNPs@PDA / PLGA nanocapsule. The method is simple, mild and environment friendly, and extra toxicity is avoided. The diameter of the prepared nanocapsule is 1-3 mm, and the nanocapsule is hollow inside and is well compatible with gold nanoparticles. The nanocapsule not only has high external ultrasound contrast imaging capacity, but also can enhance the HIFU treatment effect when used in degassed bovine liver ex vivo.

Description

technical field [0001] The invention belongs to the field of ultrasound medicine, and in particular relates to a preparation method of a multifunctional contrast agent using ultrasound-guided synergistic high-intensity focused ultrasound effect. Background technique [0002] Tumor is a malignant disease that seriously endangers human health. At present, the clinical treatment for malignant tumors is mainly surgical resection followed by radiotherapy and chemotherapy. However, this treatment has many limitations. For example, surgical resection requires high physical strength and physical fitness of the patient, and the trauma is large. It is necessary to remove the diseased tumor tissue and a large range of surrounding normal tissues at the same time, and it lacks selectivity. While inhibiting the tumor, it often has a certain impact on and damages the normal cells of the body. Often cause serious systemic side effects. [0003] As a rapidly developing non-invasive local ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K41/00A61K9/51A61K47/34A61P35/00
Inventor 范磊钱科宏奚菊群倪向颖杨莉
Owner YANGZHOU UNIV
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