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Preparation method of nanometer collagen peptide zinc chelate

A technology of nano-glue and chelated zinc, applied in the biological field, can solve problems such as the absorption efficiency of side effects, and achieve the effects of low cost, simple and mild preparation process, and good water solubility.

Active Publication Date: 2015-11-04
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Abstract
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of side effects and low absorption efficiency of traditional zinc supplements, and provide a new nano-zinc supplement-nanometer collagen peptide chelated zinc preparation method, which does not require special equipment and is easy to operate

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  • Preparation method of nanometer collagen peptide zinc chelate

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preparation example Construction

[0021] The invention discloses a preparation method of nano-collagen peptide chelated zinc, comprising the following steps:

[0022] (1) Dissolve marine biological source collagen peptides in water to make a collagen peptide aqueous solution with a concentration of 1-10 mg / ml; the marine biological source collagen peptides are collagen peptides hydrolyzed from fish skin and fish scale marine biological sources, with a molecular weight of ≤3000 Da.

[0023] (2) Add 1-10 mg / ml of protease to the above collagen polypeptide aqueous solution for enzymolysis, the enzymolysis temperature is 40-60 ℃, the pH value of the enzymolysis reaction is controlled at 6-8, and the enzymolysis is 3-6 hours; After enzymatic hydrolysis, raise the temperature to 80-90 ℃, keep the temperature for 20-30 minutes to inactivate the enzyme, and obtain a small-molecule collagen peptide aqueous solution with high metal zinc chelating activity after centrifugation; the protease used in it is trypsin and seaf...

Embodiment 1

[0028] Take 20 ml of 10 mg / ml marine biological source collagen peptide aqueous solution, add 10 mg / ml special enzyme for hydrolysis of seafood for enzymatic hydrolysis, the enzymatic hydrolysis temperature is 50°C, adjust the pH to 7.5, enzymatic hydrolysis for 6 hours, the end of enzymatic hydrolysis Afterwards, the temperature was raised to 85°C, and the enzyme was inactivated at a constant temperature for 20 min. After centrifugation at 10000 rpm, a small molecule collagen peptide aqueous solution with high metal zinc chelating activity was obtained. Prepare 4 ml of zinc chloride aqueous solution, the molar ratio of zinc ions to collagen peptide is 0.5, adjust the pH of the aqueous solution of small molecule collagen peptide to 6.0, and keep the temperature at 80 °C, add the aqueous solution of zinc chloride dropwise to the aqueous solution of small molecule collagen peptide, The whole process was continuously stirred, and after the zinc chloride aqueous solution was added...

Embodiment 2

[0030] Take 100 ml of marine biological source collagen peptide aqueous solution with a concentration of 10 mg / ml, add 1 mg / ml flavor enzyme for enzymolysis, the enzymatic hydrolysis temperature is 50°C, adjust the pH to 7.0, enzymatic hydrolysis for 6 hours, and heat up to Inactivate the enzyme at 85°C for 20 min. After centrifugation at 10000 rpm, a small molecule collagen peptide aqueous solution with high metal zinc chelating activity was obtained. Prepare 100 ml of zinc sulfate aqueous solution, the molar ratio of zinc ions to collagen peptide is 0.5, adjust the pH of the aqueous solution of small molecule collagen peptide to 6.0, and keep the temperature at 70 ℃, add the aqueous solution of zinc sulfate to the aqueous solution of small molecule collagen peptide drop by drop, the whole process Stir continuously, and after adding the zinc sulfate aqueous solution, continue stirring and heating for 120 min to obtain nano-collagen peptide chelated zinc particles with a parti...

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Abstract

The invention discloses a preparation method of nanometer collagen peptide zinc chelate of a marine organism source. The preparation method comprises the following steps: taking marine organism source collagen peptide (such as fishskin and scale hydrolyzed collagen peptide) as a raw material, preparing micromolecule collagen peptide with a high metal zinc chelating property by adopting an enzyme method, enabling the micromolecule collagen peptide and zinc salt to be subjected to a nometer chelation reaction to prepare the collagen peptide zinc chelate, wherein the collagen peptide zinc chelate is uniformly dispersed nanometer particles with the size being smaller than 1000nm, and has good water solubility. The preparation method has the advantages that the technology is simple and mild, special equipment or complicated treatment process is not needed, the cost is low, and a novel nanometer zinc supplementation preparation easy to absorb by the human body is provided.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of nano-collagen peptide chelated zinc used as a zinc supplement. Background technique [0002] Zinc is one of the essential trace elements for life. It is an important component of many enzymes, participates in the metabolism of nucleic acids and proteins, is necessary for the synthesis of collagen and keratin, and can promote bone growth and brain cell development. Zinc deficiency can easily cause symptoms such as growth retardation, mood disorders, impaired immune system, and hypogonadism. In my country, zinc deficiency is also relatively common, mainly in infants, preschool children, pregnant women and the elderly. Taking zinc supplements is a safe and effective way to improve the nutritional status of zinc-deficient people. [0003] Studies have shown that the bioavailability of zinc mainly depends on the absorption of soluble zinc in the duodenum. The bin...

Claims

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Application Information

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IPC IPC(8): A61K38/39C12P21/06A61P3/02
Inventor 郭洪辉洪专谢全灵白锴凯孙继鹏
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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