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Semisolid fungus cultivation method for nitrifying bacteria

A nitrifying bacteria and semi-solid technology, applied in the field of semi-solid culturing of nitrifying bacteria, can solve the problems of long production cycle, restricted use, high price, etc., and achieve the effects of ensuring supply, promoting growth and reproduction, and eliminating inhibition.

Inactive Publication Date: 2015-10-28
HAINAN CHIEF BIOLOGY TECHN EMPOLDER CO LTD
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] However, because nitrifying bacteria are autotrophic bacteria, their growth and reproduction are extremely slow, the production cycle is long, the stability is poor, the production efficiency is low, the production cost is high, and the price is expensive, which restricts its use. The products on the market are mainly several American companies. produced

Method used

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Embodiment Construction

[0021] The technical solutions of the present invention will be further specifically described below through examples.

[0022] The nitrifying bacteria in the present invention are essentially made up of ammonia oxidizing bacteria and nitrite oxidizing bacteria. 4 ) 2 SO 4 0.5g, NaCl 0.3g, FeSO 4 ·7H 2 O 0.03g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O0.3g, CaCl 2 7.5g, distilled water 1000mL, the main components and weight of the culture solution of nitrite oxidizing bacteria are: (analytically pure for pharmaceuticals) NaNO 2 1g, MgSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O0.01g, K 2 HPO 4 0.75g, Na 2 CO 3 (Anhydrous) 1g, NaH 2 PO 4 0.25g, distilled water 1000mL, the carrier is made of zeolite powder or bentonite raw ore, which is baked, dried and pulverized at a high temperature of 300°C to 500°C.

[0023] When cultivating, inoculate the purified ammonia oxidizing bacteria and nitrite oxidizing bacteria from the slant of the test tube into the sterilized triangular flask...

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Abstract

The invention discloses a semisolid fungus cultivation method for nitrifying bacteria. The cultivation method comprises the following steps: 1, respectively inoculating purified ammonia oxidizing bacteria and nitrite oxidizing bacteria into triangular bottles, and putting the triangular bottles in a shaking bed for 12 days' heat-insulating cultivation; 2, respectively inoculating the strains into corresponding seed tanks, pumping gas into the seed tanks, stirring, and carrying out cultivation for 12 days; 3, mixing the strains with 99 times of corresponding culture solutions, mixing the mixtures with 3 times of carriers to obtain semisolids, putting the semisolids into containers for 20 days' heat-insulating and moisture-holding cultivation; step 4, heating the containers, and removing the moisture in the cultivated semisolids with exhaust fans, and smashing the dried cultivated semisolids; step 5, mixing the two semi-finished products according to the proportion of 1:1, and mixing uniformly to obtain the finished product. The semisolid fungus cultivation method is easy to operate, low in production cost, stable in product quality, and has the advantage that the finished product contains 0.2 billion or more bacteria per gram, and is easy to store and transport.

Description

technical field [0001] The invention relates to a semi-solid culture method for nitrifying bacteria. Background technique [0002] Due to the excessive use of nitrogen fertilizers and high-density farming, the problem of nitrogen pollution in various water bodies in my country has become more and more serious. Ammonia nitrogen and nitrite nitrogen are direct or indirect factors that cause disease in various aquatic animals such as fish, shrimp, and crabs; Ammonia and nitrite in water have a strong removal ability, and are more and more widely used in sewage treatment, aquaculture, and ornamental fish industries. [0003] However, because nitrifying bacteria are autotrophic bacteria, their growth and reproduction are extremely slow, the production cycle is long, the stability is poor, the production efficiency is low, the production cost is high, and the price is expensive, which restricts its use. The products on the market are mainly several American companies. produced. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 徐桃龙谢维宣
Owner HAINAN CHIEF BIOLOGY TECHN EMPOLDER CO LTD
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