Maturative agent for hawk tea golden flower cheese and preparation method of maturative agent
A technology of cheese ripening agent and tea polysaccharide of eagle tea, which is applied in the field of new cheese ripening agent and its preparation
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[0029] 1. Preparation of tea polysaccharide concentrate of eagle tea: put 100g of eagle tea leaves in 1000ml of hot water at 90°C for 6 minutes, then add 3000ml of 95vt% ethanol, and refrigerate at 6°C for 12 hours; Min refrigerated and centrifuged for 8 min, the polysaccharide precipitate was collected, and redissolved with 5 ml sterile distilled water to obtain the eagle tea polysaccharide concentrate with a concentration of 1 g / 10 ml.
[0030] 2. Preparation of spore suspension of S. coronis
[0031] ① Domestication of S. coronis
[0032] Inoculate Saccharomyces coronis on the slant of PDA medium test tube containing tea polysaccharide concentrate of Eagle tea, the initial concentration of tea polysaccharide concentrate of Eagle tea is 0.2g / 10ml, cultivate at 25-28°C for 3-5 days, and select the colony radius The largest S. coronis was inoculated on the slant of a PDA medium test tube containing 0.5g / 10ml of eagle tea polysaccharide concentrate, cultured at 25-28°C for 3-5...
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