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A strain of Serratia for degrading triphenylmethane dye and method for degrading triphenylmethane dye

A technology of triphenylmethane and Serratia, which is applied in the directions of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of difficult treatment of polluted water bodies, and achieves low treatment costs and environmental adaptability. Strong and promising effect

Active Publication Date: 2017-11-17
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] One of the purposes of the present invention is to provide a strain of Serratia for degrading triphenylmethane dyes and a method for degrading triphenylmethane dyes

Method used

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  • A strain of Serratia for degrading triphenylmethane dye and method for degrading triphenylmethane dye
  • A strain of Serratia for degrading triphenylmethane dye and method for degrading triphenylmethane dye
  • A strain of Serratia for degrading triphenylmethane dye and method for degrading triphenylmethane dye

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1, such as figure 1 shown

[0027] Serratia WKD was inoculated in LB medium and cultured overnight at 30°C in a shaker with a rotation speed of 180rpm to obtain an overnight culture; based on the dry weight of the overnight culture, the overnight culture was 0.1g / L The material was inoculated in wastewater containing triphenylmethane dye crystal violet, and the pH was set to 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, and 11.0, and cultured with shaking at 30°C and 180rpm, and timed Sampling and determination, the sampling time is 4h, 8h and 12h respectively. After the collected samples were centrifuged at 10 000 rpm for 10 min, the supernatant was taken, and the absorbance was measured at 585 nm (λmax of crystal violet) with an Evolution 300 UV-Vis spectrophotometer to calculate the decolorization rate.

[0028]When the pH is in the range of 3.0-11.0, the decolorization rate of crystal violet can reach nearly 100% after decolorization for more than 8 hours....

Embodiment 2

[0029] Example 2, such as figure 2 shown

[0030] Serratia WKD was inoculated in LB medium and cultured overnight at 30°C in a shaker with a rotation speed of 180rpm to obtain an overnight culture; based on the dry weight of the overnight culture, the overnight culture was 0.1g / L The material was inoculated in wastewater containing triphenylmethane dye crystal violet, and the set temperatures were 5°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, 50°C, 55°C and 60°C , and shake culture under the condition of 180rpm, and take samples regularly for measurement, and the sampling time is 6h, 12h and 24h respectively. After the collected samples were centrifuged at 10 000 rpm for 10 min, the supernatant was taken, and the absorbance was measured at 585 nm (λmax of crystal violet) with an Evolution 300 UV-Vis spectrophotometer to calculate the decolorization rate.

[0031] In the temperature range of 15-50°C, the 6h decolorization rate of strain WKD to crystal violet is maintained a...

Embodiment 3

[0032] Example 3, such as image 3 shown

[0033] Serratia WKD was inoculated in LB medium, and cultured overnight at 30°C in a shaker with a rotation speed of 180rpm to obtain an overnight culture; based on the dry weight of the overnight culture, 0.1g / L of the Overnight cultures were inoculated into wastewater containing the triphenylmethane dye crystal violet at concentrations of 40mg / L, 80mg / L, 120mg / L, 160mg / L, 200mg / L, 300mg / L, 400mg / L and 500mg / L, cultured with shaking at 30°C and 180rpm, and sampled regularly for measurement, the sampling time was 4h, 8h, 12h and 24h respectively. After the collected samples were centrifuged at 10 000 rpm for 10 min, the supernatant was taken, and the absorbance was measured at 585 nm (λmax of crystal violet) with an Evolution 300 UV-Vis spectrophotometer to calculate the decolorization rate.

[0034] With the increase of the dye concentration, the decolorization rate of strain WKD to crystal violet is gradually decreasing. When the...

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Abstract

The invention discloses a Serratia strain degrading triphenylmethane dye and a method for degrading triphenylmethane dye, the strain is named Serratia sp. WKD, the preservation number is CCTCC NO: M2015178, and the preservation date As of March 29, 2015, it was deposited in the China Center for Type Culture Collection. The Serratia bacteria that degrades triphenylmethane dyes of the invention has strong environmental adaptability, has high degradation efficiency for triphenylmethane dyes malachite green and crystal violet, and has significantly better decolorization efficiency than reported strains, and has extremely high application prospects. It is broad and can solve the practical problem that the water body polluted by triphenylmethane dyes is difficult to control. Compared with the traditional physical and chemical treatment methods with high cost, low efficiency and easy to produce secondary pollution, the Serratia degraded triphenylmethane dye screened by the present invention is an environment-friendly treatment method, with low treatment cost and high efficiency, and has the advantages of low cost and high efficiency. With a certain broad spectrum, it is expected to truly realize the processing requirements of "low consumption".

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Serratia strain degrading triphenylmethane dye and a method for degrading triphenylmethane dye. Background technique [0002] Dyes are widely used in industries such as food, medicine, printing and dyeing, and cosmetics. Although there is no latest statistics on the production of dyes in the world, there are more than 100,000 types of dyes for commercial use, and the annual output of dyes in the world exceeds 7×10 5 t has been widely reported. The application of natural dyes is limited due to problems such as difficult sources and high costs. At present, the vast majority of dyes are artificially synthesized chemical dyes, most of which are aromatic compounds containing amine groups, with stable and complex structures, anti-acid, anti-alkali, anti-light, and anti-microbial properties. They are difficult to degrade and have high toxicity. The environmental residence pe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/425A62D101/20
CPCC02F3/34C02F2101/308C02F2101/34C12N1/205C12R2001/425
Inventor 都林娜李刚许方程赵宇华王阳泮琇陈文华
Owner WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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