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BpMyB106 gene in Betula platyphylla and amino acid sequence and application thereof

An amino acid and nucleotide sequence technology, used in applications, genetic engineering, plant genetic improvement, etc., to achieve the effects of increased trichome density, photosynthetic rate and growth rate, and transpiration rate.

Inactive Publication Date: 2015-10-07
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among many transcription factor families, MYB transcription factors regulate multiple life processes in plants. CCA1 (CIRCADIAN CLOCKASSOCIATED 1) in Arabidopsis and HvMCB1 and HvMCB2 in barley belong to the R1 / 2MYB family of transcription factors, which can directly regulate and participate in photosynthesis However, there are few reports on the direct regulation of photosynthesis genes by plant R2R3-MYB transcription factors

Method used

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  • BpMyB106 gene in Betula platyphylla and amino acid sequence and application thereof
  • BpMyB106 gene in Betula platyphylla and amino acid sequence and application thereof
  • BpMyB106 gene in Betula platyphylla and amino acid sequence and application thereof

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specific Embodiment approach 1

[0036] Embodiment 1: The nucleotide sequence of the birch BpMYB106 gene in this embodiment is shown in SEQ ID NO: 1 in the sequence listing.

[0037] This embodiment discloses the coding gene and application of the R2R3-MYB transcription factor BpMYB106 in birch. This embodiment uses the Agrobacterium-mediated method to overexpress the gene in birch, and the obtained birch transgenic line has increased trichome density, Characterized by increased net photosynthetic rate, transpiration rate, and growth rate. Using digital expression profiling technology to analyze the differential genes of wild-type and transgenic birch, it was found that some genes directly related to photosynthesis in the transgenic birch were up-regulated, and the yeast single hybrid technology verified that the BpMYB106 transcription factor can combine photosynthesis-related cis The action element, and then directly regulate the photosynthetic gene, so that the photosynthetic rate and growth rate of birch a...

specific Embodiment approach 2

[0038] Specific embodiment 2: The amino acid sequence of the gene encoding birch BpMYB106 in this embodiment is shown in SEQ ID NO: 2 in the sequence listing.

specific Embodiment approach 3

[0039] Specific embodiment three: the application of birch BpMYB106 gene in this embodiment refers to the application in increasing the expression abundance of photosynthesis-related genes.

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Abstract

The invention relates to and aims at providing a BpMyB106 gene in Betula platyphylla and an amino acid sequence and application thereof. A nucleotide sequence of the BpMyB106 gene in Betula platyphylla is show in a sequence list SEQ ID NO.1; the amino acid sequence thereof is shown in a sequence list SEQ ID NO.2. The BpMyB106 gene helps improve expressive abundance of photosynthesis related genes. The yeast one-hybrid technique shows that a BpMyB106 transcription factor is capable of directly controlling photosynthetic genes to increase the photosynthetic speed and growth speed of Betula platyphylla by the combination with photosynthesis related cis-acting elements; a new variety of Betula platyphylla with the trans-BpMYB106 gene, which is high in luminous effect and growth speed, can be cultivated with the BpMyB106 gene; the BpMyB106 gene is applicable to the field of molecular breeding of trees.

Description

technical field [0001] The invention relates to a birch gene, its amino acid sequence and application. Background technique [0002] With the development of society and economy and the continuous consumption of energy, the energy stored in the earth is increasingly depleted. The woody tissue of trees constitutes 20% of the global terrestrial carbon storage, and these carbons are derived from the products of photosynthesis. Therefore, increasing the carbon sequestration of woody plants, the production of biological materials and the application efficiency of biofuels is an important way to solve the problem of renewable energy, and improving the photosynthesis of forest trees has become an effective means. [0003] Plant photosynthesis generally has low efficiency, and the efficiency of plants converting light energy for plant growth in photosynthesis is only 2% to 4%. There is a big gap between the actual photosynthetic efficiency and the potential photosynthetic efficiency...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
Inventor 李成浩周晨光王玉成
Owner NORTHEAST FORESTRY UNIVERSITY
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