Specific primers for amplifying plecoptera insect mitochondrion COI (cytochrome c oxidase I) gene
A technology of Anoptera and mitochondria, which is applied in the direction of genetic engineering, plant gene improvement, application, etc., to achieve the effect of reducing experiment cost, improving accuracy, and shortening experiment cycle
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[0013] The amplification primer screening method of the Coeloptera COI gene of the present invention is divided into two steps: 1. Based on the sequence of the COI gene in the mitochondrial genome sequence of the Cooptera insect that has been measured, find out the COI gene by comparison The relatively conserved sequence fragments at both ends do not rely on the existing universal primers for insects, and design special primers for the order of the order; 2. Amplify the COI gene of the insects of the order of the order with the synthesized primers, and test its generality in the order of the order. 3. Many pairs of primers have been designed in the process, and the verification of experimental results shows that the primers of the present invention are optimal
[0014] Below in conjunction with example the invention is further described:
[0015] 1. Sample preparation: The samples were all insects of different families of the order Coptera stored in the specimen room of the In...
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