Acclimatization transition method for improving transplanting survival rate of tissue culture seedlings of nicotiana tabacum K326

Abstract

The invention discloses an acclimatization transition method for improving the transplanting survival rate of tissue culture seedlings of nicotiana tabacum K326. Three sections of acclimatization are performed on rooted tissue culture seedlings of the nicotiana tabacum K326, and the pot soil is heated so as to be disinfected; the culture media of roots of the tissue culture seedlings are removed; complete seedlings are soaked in carbendazim liquid to be disinfected, and then the disinfected seedlings are washed by water; the washed tissue culture seedlings are transplanted in the disinfected pot soil; after growing, surviving and strengthening indoors, the tissue culture seedlings are separated and transplanted in non-disinfected pot soil, and after growing, the tissue culture seedlings are placed at an outdoor shade for continuous growth for weeks; finally, the seedlings are moved to normal open air, and the seedlings are conventionally watered and fertilized, so that the acclimatization transition of the tissue culture seedlings of the nicotiana tabacum K326 is completed. Through the adoption of the acclimatization transition method disclosed by the invention, the survival rate of transgenic tissue culture seedlings of nicotiana tabacum after the tissue culture seedlings are transplanted outdoors is greatly improved, and the survival rate can reach 96.7-100%; the workload is reduced; the time is shortened; the energy consumption is lowered; the cost is reduced. Through the acclimatization transition method disclosed by the invention, the acclimatization time for seedlings can be shortened, and the steps of acclimatization, disinfection, transplanting and potting and the like can be completed within one week.

Description

technical field [0001] The invention relates to a seedling hardening transition method for improving the transplanting survival rate of tobacco K326 tissue cultured seedlings, and belongs to the technical field of plant breeding and cultivation. Background technique [0002] tobacco( Nicotiana tabacum ) is an important annual or perennial economic crop of Nicotiana in the family Solanaceae. Tobacco has become an important model plant in the field of plant genetic engineering due to its easy tissue culture and high transformation efficiency. Tobacco K326 was introduced from the United States to Yunnan, my country in 1985. Its advantage is that its aroma quality and aroma are superior to other tobacco varieties. It is currently the variety with the largest planting area in my country, and it is also widely used as an important model in the verification of plant gene functions. one of the plants. [0003] Tobacco, as a model plant, is widely used in experiments for the functio...

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Problems solved by technology

[0004] At present, in the general outdoor hardening transition process of tobacco K326 tissue culture seedlings, it is often only a simple and extensive indoor hardening process, and only a certain concentration of fungicides such as carbendazim are used for a certain period of time. In the stage of indoor seedling hardening, the tissue cultured seedlings did not go through a continuous and gradual transitional hardening process, or fungicides such as carbendazim were not effective in killing pathogens in the external environment such as the cultivation soil, or due to poor operation during the transplanting process. Planning and late management of tobacco tissue cultured seedlings just after they are transplanted into pots, so in the past, tobacco transgenic tissue cultured seedlings often died after being transferred from the tissue culture room, during the transition process of seedling hardening and after transplanting The survival rate is too high, the survival rate does not exceed 70%, and in severe cases, the entire batch of seedlings will die after transplanting.

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