A kind of method for microbial treatment of tobacco stems to enhance aroma
A technology for microbial treatment and aroma enhancement, which is applied in the treatment of tobacco, tobacco, food science, etc. It can solve the problems of astringent taste, limit the proportion of stalks, and less aroma, and achieve excellent effect of aroma enhancement, improve utilization and cost. cheap effect
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Embodiment 1
[0020] A method for microbial treatment of tobacco stems to enhance aroma, characterized in that the steps are as follows:
[0021] Get Micrococcus acidophilus and Bacillus subtilis to inoculate in an additional 200ml LB liquid culture medium containing 0.2% trehalose by weight according to the inoculum size of 0.1% and 0.15% respectively;
[0022] Shake at 37°C at 160r / min to OD value = 2.2; centrifuge at 3800rpm / min for 8min, then wash the precipitate with sterile water, centrifuge, shake evenly with 20mL of sterile water, and dilute 15% when used. times, and adding pectinase is the bacterial agent for treatment;
[0023] Weigh the cut stems, spray 0.12 times the weight of the cut stems on the cut stems, seal it, and keep it at a constant temperature for 5 hours at 30°C, then dry the cut stems, and then put the cut stems at a constant temperature of 20°C and a relative humidity of 50%. Equilibrate in a constant humidity chamber for 48 hours.
[0024] Prepare each liter of ...
Embodiment 2
[0030] A method for microbial treatment of tobacco stems to enhance aroma, characterized in that the steps are as follows:
[0031] Get Micrococcus acidophilus, and Bacillus subtilis is inoculated in 200ml of LB liquid medium that contains 0.2% trehalose by weight percentage according to the inoculum size of 0.1% and 0.15% respectively;
[0032] Shake at 37°C at 160r / min to OD value = 2.2; centrifuge at 3800rpm / min for 8min, then wash the precipitate with sterile water, centrifuge, shake evenly with 20mL of sterile water, and dilute 15% when used. times, and adding pectinase is the bacterial agent for treatment;
[0033] Weigh the cut stems, spray 0.15 times the weight of the cut stems on the cut stems, seal it, and keep it at a constant temperature for 5 hours at 30°C, then dry the cut stems, and put the cut stems at a constant temperature of 22°C and a relative humidity of 60%. Equilibrate in a constant humidity chamber for 48 hours.
[0034] Prepare each liter of LB mediu...
Embodiment 3
[0040] A method for microbial treatment of tobacco stems to enhance aroma, characterized in that the steps are as follows:
[0041] Get Micrococcus acidophilus, and Bacillus subtilis is inoculated in 200ml of LB liquid medium that contains 0.2% trehalose by weight percentage according to the inoculum size of 0.1% and 0.15% respectively;
[0042] Shake at 37°C at 160r / min to OD value = 2.2; centrifuge at 3800rpm / min for 8min, then wash the precipitate with sterile water, centrifuge, shake evenly with 20mL of sterile water, and dilute 15% when used. times, and adding pectinase is the bacterial agent for treatment;
[0043] Weigh the cut stems, spray 0.13 times the weight of the cut stems on the cut stems, seal it, and keep it at a constant temperature for 5 hours at 30°C, then dry the cut stems, and put the cut stems at a constant temperature of 21°C and a relative humidity of 55%. Equilibrate in a constant humidity chamber for 48 hours.
[0044] Prepare each liter of LB mediu...
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