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Nucleic acid aptamer probe, device and application for tumor cell detection

A nucleic acid aptamer and tumor cell technology, applied in the field of devices and nucleic acid aptamer probes for tumor cell detection, can solve the problems of high cost, time-consuming, complicated operation, etc., and achieve low cost, low instrument requirements, and universal adaptable effect

Active Publication Date: 2018-01-16
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the current conventional detection methods of tumor cells, especially circulating tumor cells and non-solid tumor cells, there are problems such as complicated operation, long time-consuming and high cost.

Method used

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  • Nucleic acid aptamer probe, device and application for tumor cell detection
  • Nucleic acid aptamer probe, device and application for tumor cell detection
  • Nucleic acid aptamer probe, device and application for tumor cell detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] see figure 1 : A kind of device that is used for tumor cell detection of the present invention, comprises nucleic acid aptamer probe and carrier, wherein nucleic acid aptamer probe comprises immobilized probe, recognition probe, and immobilized probe 3' end is modified with biotin, The 5' end is modified with a quenching group BHQ1, and the 3' end of the recognition probe is modified with a fluorescent group FAM; the carrier is a superparamagnetic magnetic particle coated with streptavidin.

[0039] The nucleotide sequence of the fixed probe has the nucleotide sequence described in SEQ ID NO.1; specifically:

[0040]Immobilized Probe-CEM: 5'-BHQ1-TCT AAC CGT ATT TTT TTT TT -biotin-3'.

[0041] The nucleotide sequence of the recognition probe is the nucleotide sequence described in SEQ ID NO.2; specifically:

[0042] Recognition probe-CEM: 5'-ATC TAA CTG CTG CGC CGC CGG GAA AAT ACT GTA CGG TTAGA-FAM-3'.

[0043] The TCT AAC CGTA fragment of the immobilized probe-CEM i...

Embodiment 2

[0063] A device for tumor cell detection of the present invention comprises a nucleic acid aptamer probe and a carrier, wherein the nucleic acid aptamer probe includes an immobilized probe and a recognition probe, and the 3' end of the immobilized probe is modified with biotin, The 5' end of the recognition probe is modified with a fluorescent group FAM; the carrier is a superparamagnetic particle coated with streptavidin.

[0064] The nucleotide sequence of the fixed probe has the nucleotide sequence described in SEQ ID NO.3; specifically:

[0065] Immobilized Probe-7721: 5'-CGC GCG CTT TTT TTT TTT -biotin-3'.

[0066] The nucleotide sequence of the recognition probe is the nucleotide sequence described in SEQ ID NO.4; specifically:

[0067] Recognition probe-7721: 5'-FAM-AAA GCG CGC GCG CGC ATA GCG CGC TGA GCT GAA GATCGT ACC GTG AGC GCG CT-3'.

[0068] The CGC GCG CTT T fragment of the immobilized probe is hybridized with the AAA GCG CGC G fragment in the recognition probe...

Embodiment 3

[0088] see Figure 7 : A device for tumor cell detection of the present invention, comprising a nucleic acid aptamer probe and a carrier, wherein the nucleic acid aptamer probe includes a fixed probe and a recognition probe, and the 3' end of the fixed probe is modified with biotin , the identification probe carries a medical contrast agent; the carrier is a microfluidic chip (the carrier can also be artificial blood vessels, vascular stents and other microchannels). The medical contrast agent used in this embodiment is pyropheophorbide-a, and the microfluidic chip is a microfluidic chip bonded between PDMS polymer and glass.

[0089] The nucleotide sequence of the fixed probe has the nucleotide sequence described in SEQ ID NO.3; specifically:

[0090] Immobilized Probe-7721: 5'-CGC GCG CTT TTT TTT TTT -biotin-3'.

[0091] The nucleotide sequence of the recognition probe is the nucleotide sequence described in SEQ ID NO.4; specifically:

[0092] Recognition probe-7721: 5'-F...

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Abstract

The invention discloses a nucleic acid aptamer probe for tumor cell detection, a device and its application, wherein the nucleic acid aptamer probe includes an immobilized probe and a recognition probe capable of binding to tumor cells; the immobilized probe and The recognition probe part is hybridized and complementary; the device includes a nucleic acid aptamer probe and a carrier, and the immobilized probe of the nucleic acid aptamer probe is cross-linked on the surface of the carrier through covalent or non-covalent cross-linking. The nucleic acid aptamer probe instrument of the present invention has low requirements, strong universality, low cost, simple operation and easy popularization, and can be applied to the detection, tracing and killing of tumor cells.

Description

technical field [0001] The invention relates to the technical field of tumor diagnosis and treatment, in particular to a nucleic acid aptamer probe, device and application for tumor cell detection. Background technique [0002] Tumor is the normal regulation of the growth of cells in local tissues at the gene level under the action of various tumorigenic factors, resulting in clonal abnormal proliferation. Clinically, tumors can be divided into solid tumors and non-solid tumors. Solid tumors and tangible tumors can be palpable through clinical examinations such as CT scans, B-ultrasound and other imaging methods or palpable tangible masses. Non-solid tumors such as leukemia in blood diseases cannot be directly diagnosed by palpation or conventional imaging methods. Tumors not only grow out of control, but also locally invade the surrounding normal tissues and even metastasize to other parts of the body through the internal circulatory system or lymphatic system, seriously t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12M1/00
Inventor 王柯敏陈南迪羊小海翦立新王青
Owner HUNAN UNIV
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