Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bovine influenza virus compositions

A technology of influenza virus and composition, applied in the field of microbiology and immunology, can solve problems such as mild upper respiratory tract infection or infection is not obvious

Inactive Publication Date: 2015-08-19
ZOETIS SERVICE LLC
View PDF34 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, influenza type C viruses are rare compared with types A or B, and are not known to cause mild upper respiratory tract infections or infections in humans

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bovine influenza virus compositions
  • Bovine influenza virus compositions
  • Bovine influenza virus compositions

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0133] Example 1: Virus Isolation

[0134] Nasal swab bank from bovine respiratory disease cases for virus isolation. Primary isolation of virus was done on HRT-18G cells. Subsequent passages on HRT-18G cells resulted in high titer virus (~1x10 8 TCID 50 / mL) for larger formulations. No cytopathic effect (CPE) was observed on infected HRT-18G cells; however, giant cells were occasionally present, indicative of virus-induced cell fusion. The presence of virus was confirmed by indirect immunofluorescent antibody (IFA) staining using pooled convalescent bovine sera as the primary antibody source followed by anti-bovine FITC-conjugated secondary antibody. The virus was initially identified as isolate #14. No exotic viral pathogens of bovine origin were identified in the culture medium.

example 2

[0135] Example 2: Sensitivity of Multiple Cell Types

[0136] Various cell lines were evaluated for their susceptibility to the isolated virus. Virus titrations were also performed on culture fluids harvested from infection of various productive cells. CPE was generally not observed, except on NLST (porcine testis) cells.

[0137] Table 1. Cell lines tested for virus growth and yield.

[0138] cell type

[0139] Trypsin treatment does not appear to be necessary for virus propagation. Furthermore, upon further investigation, it was concluded that although the virus did infect some cell lines, it was not a productive infection (sometimes referred to as "abortive infection"). HRT-18G, NLST, MA-104 and BK-6 cells were productively infected, with HRT-18G and BK-6 cells obtaining the highest titers.

example 3

[0140] Example 3: Hemagglutination assay

[0141] Standard hemagglutination (HA) tests were performed using red blood cells from roosters as well as guinea pigs. Results (not shown) indicated that the bovine virus was positive for HA and high titers were obtained in the HA test.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

An influenza C virus has been isolated from a bovine species. Influenza C virus polynucleotides and polypeptides have also been identified. Immunogenic compositions are also described, as well as diagnostic kits and methods of detection.

Description

technical field [0001] The present invention relates to the fields of microbiology and immunology, and in particular to viruses and immunogenic compositions comprising the same. Specifically, it concerns influenza C viruses isolated from cattle. Also disclosed herein are influenza C virus polynucleotides and polypeptides. Finally, the invention discloses a diagnostic kit and a detection method. Background technique [0002] The bovine respiratory disease (BRD) complex is the most significant health problem in the beef cattle industry. In 1991, an estimated $624 million in losses occurred due to treatment costs, lost production and fatalities. The BRD complex is a multifactorial infection with many contributing pathogens, both viral and bacterial. [0003] Viruses with enveloped single-stranded RNA containing negative-sense genomes are classified as groups with non-segmented genomes (Paramyxoviridae, Rhabdoviridae) or groups with segmented genomes (Orthomyxoviridae, Bunia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/11A61K39/145C07K16/10
CPCG01N2469/10A61K39/12C12N2760/16334A61K2039/543G01N2333/11A61K39/145C07K14/005G01N33/56983A61K2039/5254A61K2039/5252C07K16/1018A61K2039/505C12N7/00C12N2760/16321C12N2760/16322
Inventor S-K·W·韦尔奇
Owner ZOETIS SERVICE LLC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com