Application of Lunasin polypeptide in aspect of preparing substance with weight-reducing activity
An active substance and aspect technology, which is applied in the application field of Lunasin polypeptide in the preparation of active substances with weight loss, can solve the problems of lack of new activity research, and achieve the effects of reducing accumulation and inhibiting mRNA expression.
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Embodiment 1
[0025] The effect of Lunasin on the activity of 3T3-L1 adipocytes was determined by MTT method:
[0026] After 3T3-L1 preadipocytes were recovered and passaged, they were seeded in a 96-well plate at a density of 10,000 cells / ml. After 24 hours, the medium containing 800 μg / mL Lunasin was replaced, and a cell-free and lunasin-free blank group and a Lunasin-free negative control were set. Group. After 48 hours, 20 μL of MTT solution (1 mg / mL) was added to each well, and incubated anaerobically at 37°C for 4 hours. After MTT was removed, 200 μL of DMSO (dimethyl sulfoxide) was used to dissolve the crystals, and the absorbance was measured at 570 nm. The blank group was used for zero adjustment, and it was found that, compared with the negative control group, after adding 800 μg / mL Lunasin, the cell viability was significantly reduced.
Embodiment 2
[0028] The effect of Lunasin on the activity of 3T3-L1 adipocytes was determined by MTT method:
[0029] After 3T3-L1 preadipocytes were recovered and passaged, they were seeded in a 96-well plate at a density of 10,000 cells / ml. After 24 hours, the medium containing 400 μg / mL Lunasin was replaced, and a cell-free and lunasin-free blank group and a Lunasin-free negative control were set. Group. After 48 hours, 20 μL of MTT solution (1 mg / mL) was added to each well, and incubated anaerobically at 37°C for 4 hours. After MTT was removed, 200 μL of DMSO was used to dissolve the crystals, and the absorbance was measured at 570 nm. The blank group was used for zero adjustment, and it was found that, compared with the negative control group, after adding 400 μg / mL Lunasin, the cell viability was significantly reduced.
Embodiment 3
[0031] The effect of Lunasin on the activity of 3T3-L1 adipocytes was determined by MTT method:
[0032] After 3T3-L1 preadipocytes were recovered and passaged, they were seeded in a 96-well plate at a density of 10,000 cells / ml. After 24 hours, the medium containing 200 μg / mL Lunasin was replaced, and a cell-free and lunasin-free blank group and a negative control without Lunasin were set. Group. After 48 hours, 20 μL of MTT solution (1 mg / mL) was added to each well, and incubated anaerobically at 37°C for 4 hours. After MTT was removed, 200 μL of DMSO was used to dissolve the crystals, and the absorbance was measured at 570 nm. The blank group was used for zero adjustment, and it was found that, compared with the negative control group, after adding 200 μg / mL Lunasin, the cell viability was significantly reduced.
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