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Application of Lunasin polypeptide in aspect of preparing substance with weight-reducing activity

An active substance and aspect technology, which is applied in the application field of Lunasin polypeptide in the preparation of active substances with weight loss, can solve the problems of lack of new activity research, and achieve the effects of reducing accumulation and inhibiting mRNA expression.

Inactive Publication Date: 2015-08-19
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research on the biological activity of Lunasin focuses on the above three aspects, while the research on its new activity is relatively scarce

Method used

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  • Application of Lunasin polypeptide in aspect of preparing substance with weight-reducing activity
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  • Application of Lunasin polypeptide in aspect of preparing substance with weight-reducing activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The effect of Lunasin on the activity of 3T3-L1 adipocytes was determined by MTT method:

[0026] After 3T3-L1 preadipocytes were recovered and passaged, they were seeded in a 96-well plate at a density of 10,000 cells / ml. After 24 hours, the medium containing 800 μg / mL Lunasin was replaced, and a cell-free and lunasin-free blank group and a Lunasin-free negative control were set. Group. After 48 hours, 20 μL of MTT solution (1 mg / mL) was added to each well, and incubated anaerobically at 37°C for 4 hours. After MTT was removed, 200 μL of DMSO (dimethyl sulfoxide) was used to dissolve the crystals, and the absorbance was measured at 570 nm. The blank group was used for zero adjustment, and it was found that, compared with the negative control group, after adding 800 μg / mL Lunasin, the cell viability was significantly reduced.

Embodiment 2

[0028] The effect of Lunasin on the activity of 3T3-L1 adipocytes was determined by MTT method:

[0029] After 3T3-L1 preadipocytes were recovered and passaged, they were seeded in a 96-well plate at a density of 10,000 cells / ml. After 24 hours, the medium containing 400 μg / mL Lunasin was replaced, and a cell-free and lunasin-free blank group and a Lunasin-free negative control were set. Group. After 48 hours, 20 μL of MTT solution (1 mg / mL) was added to each well, and incubated anaerobically at 37°C for 4 hours. After MTT was removed, 200 μL of DMSO was used to dissolve the crystals, and the absorbance was measured at 570 nm. The blank group was used for zero adjustment, and it was found that, compared with the negative control group, after adding 400 μg / mL Lunasin, the cell viability was significantly reduced.

Embodiment 3

[0031] The effect of Lunasin on the activity of 3T3-L1 adipocytes was determined by MTT method:

[0032] After 3T3-L1 preadipocytes were recovered and passaged, they were seeded in a 96-well plate at a density of 10,000 cells / ml. After 24 hours, the medium containing 200 μg / mL Lunasin was replaced, and a cell-free and lunasin-free blank group and a negative control without Lunasin were set. Group. After 48 hours, 20 μL of MTT solution (1 mg / mL) was added to each well, and incubated anaerobically at 37°C for 4 hours. After MTT was removed, 200 μL of DMSO was used to dissolve the crystals, and the absorbance was measured at 570 nm. The blank group was used for zero adjustment, and it was found that, compared with the negative control group, after adding 200 μg / mL Lunasin, the cell viability was significantly reduced.

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Abstract

The invention discloses application of Lunasin polypeptide in the aspect of preparing substance with weight-reducing activity. Lunasin is substance with weight-reducing activity and is prepared by inhibiting differentiation of mouse preadipocyte (3T3-L1) through a PPAR gamma passage. The application includes: using an MTT method to detect influence, on 3T3-L1 adipocyte activity, of Lunasin at different dosages; detecting inhibiting effect, on adipocyte differentiation, of Lunasin at different dosages. Compared with current study conditions of Lunasin, the application has the advantages that the application shows that Lunasin can inhibit differentiation of insulin-induced 3T3-L1 cells, reduce accumulation of lipid droplets in lipid and inhibit mRNA expression of PPAR gamma, Lunasin presents dosage dependence, and effect of Lunasin with dosage higher than 50ug / mL is better than that of rosiglitazone of 5ug / mL; the application shows that Lunasin has potential weight-reducing effect.

Description

technical field [0001] The present invention relates to the application of new physiologically active substances of Lunasin polypeptides, in particular to the application of Lunasin polypeptides in the preparation of active substances for weight loss, and in particular to the preparation of Lunasin by inhibiting the differentiation of mouse preadipocytes (3T3-L1) through the PPARγ pathway. It has applications in the aspect of weight loss active substances. Background technique [0002] Lunasin is a polypeptide containing 43 amino acids that was first isolated and identified from soybeans. Animal experiments have shown that Lunasin will not be completely digested and degraded in the gastrointestinal system, but can be completely absorbed by the body. Lunasin is considered to be a brand-new peptide with cancer prevention effect. Lunasin can also reduce the body's serum total cholesterol level and LDL-C, and has a significant lipid-lowering effect. In addition, it has been rep...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P3/04
Inventor 任贵兴朱莹莹么杨
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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