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Application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease

A technology of B-1619, amylolytic spores, applied in the fields of application, nematicides, chemicals for biological control, etc., can solve the problems that vegetable root-knot nematode has not been reported, and achieve good control effect, persistent occurrence and The effect of being popular and not polluting the environment

Inactive Publication Date: 2015-08-19
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bacillus amyloliquefaciens is the dominant biological population of soil and plant micro-ecological flora, non-toxic, non-pathogenic, safe for humans and animals; because it can produce spores resistant to heat, drought, ultraviolet rays and organic solvents, it is an ideal Many strains with excellent properties have been successfully applied to the biological control of plant diseases. For example, the Chinese patent No. "201210208366.9" discloses a Bacillus amyloliquefaciens B- 1619, and applied it to the control of soil-borne diseases of Solanaceae vegetables, but there is no report on the use of Bacillus amyloliquefaciens to control vegetable root-knot nematodes

Method used

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  • Application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Embodiment 1 Preparation of Bacillus amyloliquefaciens B-1619 water-dispersible granules

[0014] Raw materials: by mass percentage, binder magnesium aluminum silicate 6%, dispersant NNO 4%, disintegrant ammonium sulfate 10%, wetting agent sodium lauryl sulfate, Bacillus amyloliquefaciens B-1619 biocontrol bacteria Bacteria liquid 30%, the remainder is kaolin.

[0015] Preparation method: Put all the raw materials except the Bacillus amyloliquefaciens B-1619 biocontrol bacteria liquid into the tank mixer (DH-200) at a speed of 24rpm, and mix evenly for 9-10 minutes; then put the materials into Double-screw mixer (DSH-1M 3 ), add the Bacillus amyloliquefaciens B-1619 biocontrol bacteria liquid, the speed is 1460rpm, stir and mix for 2-3 minutes; then put the mixed material into the rotary granulator (ZLB-300) for extrusion Compression granulation; the particle size of the granules is 1.0-1.5mm. After granulation, put them into the fluidized bed granulation coating ...

Embodiment 2

[0018] Example 2 Application of Bacillus amyloliquefaciens B-1619 water-dispersible granules in the control of tomato root-knot nematode

[0019] From August 2014 to January 2015, a demonstration test of Bacillus amyloliquefaciens biocontrol bacteria B-1619 water-dispersible granules to control tomato root-knot nematode was carried out in Qianjin Village, Tangzhang Town, Tongshan District, Xuzhou City.

[0020] Experimental group: using the Bacillus amyloliquefaciens B-1619 water-dispersible granules obtained in Example 1;

[0021] Control group: the drug used was abamectin;

[0022] Blank control: no medicine was used.

[0023] 1. Experimental method:

[0024] The method of the experimental group is:

[0025] (1) The facility vegetable seedlings used for transplanting and setting seedlings must be plant seedlings that use matrix plugs to raise seedlings, and plant seedlings that have been infested by root-knot nematodes are strictly prohibited;

[0026] (2) Open the pla...

Embodiment 3

[0045] Example 3 Application of Bacillus amyloliquefaciens B-1619 water-dispersible granules in the control of cucumber root-knot nematode

[0046] From February 2014 to July 2014, a demonstration test of Bacillus amyloliquefaciens biocontrol bacteria B-1619 water-dispersible granules to control cucumber root-knot nematode was carried out in Qianjin Village, Tangzhang Town, Tongshan District, Xuzhou City. The experimental method and Example 2 Same, the experimental results are shown in Table 2:

[0047] Table 2 The effect of Bacillus amyloliquefaciens biocontrol bacteria B-1619 water dispersible granules on controlling cucumber root-knot nematode

[0048]

[0049] The results of the greenhouse test showed that the Bacillus amyloliquefaciens biocontrol bacteria B-1619 water-dispersible granules had a good control effect on cucumber root-knot nematodes.

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Abstract

The invention discloses an application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease. A Bacillus amyloliquefaciens B-1619 water dispersible granule with the bacterium content of at least 1.2*10<8>cfu / mL is used to control root-knot nematode disease of protected vegetables, and the water dispersible granule comprises 6% of a binder, 4% of a dispersant, 10% of a disintegrating agent, 2% of a wetting agent, 20% of a Bacillus amyloliquefaciens B-1619 biocontrol bacterium liquid, and the balance of a filler. The Bacillus amyloliquefaciens is colonized to the body surface or the internal of plant rhizosphere, and competes with root-knot nematodes on nutrition surrounding the plants to deteriorate the survival environment of the root-knot nematodes, so the Bacillus amyloliquefaciens B-1619 has the advantages of good control effect, non-toxicity, no pathogenicity, safety to human and animals, no pollution to environment, and effective and lasting control of the generation and popularization of the root-knot nematodes of the protected vegetables.

Description

technical field [0001] The invention relates to the field of biological control, in particular to the application of bacillus amyloliquefaciens B-1619 in preventing and controlling root-knot nematode diseases. Background technique [0002] Facility vegetables refer to vegetables grown in greenhouses, greenhouses, small sheds, or mulched with plastic film, and have become a pillar industry in modern agricultural production and the main way for farmers to increase their income. In recent years, with the increase of the planting area of ​​protected vegetables and successive years of planting, root-knot nematodes in protected vegetables have been increasing year by year, and have risen to become an important disease in vegetable production. Root-knot nematode (Meloidogyne) is a highly specialized omnivorous phytopathogenic nematode, which can infect more than 3000 kinds of plants and harm a variety of vegetable crops, such as cucumber, loofah, cowpea, kidney bean, eggplan...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N25/14A01P5/00
Inventor 陈志谊蒋盼盼陆凡明亮刘邮洲
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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