Method for breeding wide-compatibility indica male sterile line with dian-type cytoplasm

Abstract

The invention relates to a method for breeding a wide-compatibility indica male sterile line with dian-type cytoplasm. The method comprises the following steps: selecting a good-quality japonica rice maintainer line material with a wide-compatibility gene, a short straw, disease resistance as a female parent, hybridizing with existing indica and japonica, selecting a middle partial-indica type short-straw and premature maintainer line material as a male parent to hybridize, and carrying out back-crossing on the hybrid F1 generation with the male parent; selecting partial-indica ideal plant type from back-crossed middle generation, and transforming with a dian-type sterile line material; selecting all-sterile cells or cells with high sterile rate from transformation cells of the dian-type cytoplasm, and carrying out transformation by adopting the corresponding indica maintainer line material which conforms to the breeding targets and requirements as a key point; carrying out build-up surface test-crossing on a stable sterile line material, selecting highly-dominant hybrid rice to combine, and carrying out seed reproduction and seed production of the cells. The method is high and stable in transformation speed; and the obtained sterile line is wide in build-up surface, high in seed reproduction and seed production yield and good in stress resistance.

Description

technical field [0001] The invention relates to the field of rice breeding, in particular to a method for breeding wide-compatibility indica-type sterile lines by using Dian-type cytoplasm. Background technique [0002] Three-line hybrid rice is a great achievement in rice breeding and promotion. The so-called three-line is: (1) male sterile line: the pistil develops normally, but the development of the stamen degenerates or aborts, and cannot self-pollinate and bear fruit; (2) maintain Line: the pistil of the male sterile line develops normally, and its pollen is given to the pistil of the male sterile line, not only can form seeds, but also can still obtain male sterile plants after sowing; (3) restorer line: its pollen is given to the pistil of the male sterile line, After the resulting seeds are sown, the grown plants regain their fertility. [0003] Rice is the same as other crops, the strength of heterosis is subject to the genetic molecular level of parental genes. ...

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for breeding a wide-compatibility indica-type sterile line with Dian-type cytoplasm in view of the prior art. Physiological and reproductive barriers such as insufficiency, insufficient grains, etc., so as to breed indica-type sterile lines with high yield, good resistance, and high quality, laying the foundation for the cultivation of super-high-yield hybrid rice combinations

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