Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Wrinkle-removing short peptide and its preparation method

A technology of short wrinkle peptides and short peptides, which is applied in the field of new anti-wrinkle short peptides and its preparation, can solve the problems of high cost, limited wide use, limited chemical synthesis, etc., and achieves low production cost, easy promotion, and good water solubility Effect

Active Publication Date: 2018-11-02
SHANXI JINBO BIO PHARMA CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current preparation methods of hexapeptide are still limited to chemical synthesis, and its high cost limits the widespread use of such products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Wrinkle-removing short peptide and its preparation method
  • Wrinkle-removing short peptide and its preparation method
  • Wrinkle-removing short peptide and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] Specific embodiments of the present invention will be described in detail below.

[0035] A preparation method for wrinkle-removing short peptide, comprising the steps of:

[0036] (1) Construction of Escherichia coli genetically engineered bacteria

[0037] 1.1. In order to obtain the wrinkle-removing short peptide rE14 after enzyme digestion, the amino acid sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro-Glu-Glu-Met-Gln-Arg-Arg is specially designed as the basic repeating unit L14, The protein sequence pL14-n was constructed.

[0038] The number of repeats of this unit is directly proportional to the yield of the final short peptide. Usually, 1-100 recombination units can be used for construction, and the target protein sequence constructed in this way (LEVLFQGPEEMQRR) n It is abbreviated as pL14-n, where n is any value from 1 to 100; experiments have shown that when n=9, the yield of wrinkle-removing short peptide rE14 is higher.

[0039] In this example, 9 repeating un...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of a recombinant protein in the field of genetic engineering and particularly relates to a novel wrinkle-eliminating oligopeptide and a preparation method thereof. The invention discloses the wrinkle-eliminating oligopeptide named as E14 composed of 14 amino acids and also discloses a process for fermenting by virtue of escherichia coil to industrially prepare recombinant E14 (short for rE14) on a large scale. Proven through detecting by using various biological means, the production process of rE14 is simple, low in cost, high in purity and stability, capable of bringing nutrient skin anti-aging products to the daily life of common people and wide in market application prospect.

Description

technical field [0001] The invention relates to the design and preparation method of recombinant protein in the field of genetic engineering, in particular to a novel wrinkle-removing short peptide and its preparation method. Background technique [0002] The skin of humans and higher animals consists of epidermis, dermis, and subcutaneous tissue. The epidermis has no blood vessels, and the energy and nutrition of the cells must be provided by the microcirculation of the dermis. The dermis is the lifeblood of the skin. It is a layer of dense, tough and elastic tissue, mainly composed of fibrous components (collagen fibers, elastic fibers and reticular fibers), glycoproteins, glucosamine and hyaluronic acid. [0003] Human studies have confirmed that wrinkles are associated with overstimulation of facial muscle fibers. Therefore, it is theoretically possible to reduce wrinkles by directly reducing muscle contraction. Muscle contraction is regulated by neurotransmitters, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12P21/02C07K1/22A61K8/64C12R1/19
Inventor 杨霞赵绵松
Owner SHANXI JINBO BIO PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com