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Trichome specific promoters

A promoter and carrier technology, applied in the direction of introducing foreign genetic material using vectors, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problem of low activity, no trichome-specificity and high promoter activity Combination and other issues

Inactive Publication Date: 2015-08-05
凯金公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these promoters have shown strict trichome-specificity, their activity is relatively low
[0011] Although trichome-specific promoters have been isolated, none of them showed the combination of trichome-specificity and high promoter activity

Method used

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Embodiment Construction

[0069] The use of constitutive promoters such as the CaMV 35S promoter (single 35S promoter, described by Franck et al., 1980, Cell 21, 285-294) is not considered for genes requiring regulation at a specific tissue level (e.g. phloem or hair genes in the body), or the expression of genes that are regulated only under specific conditions (eg, stress-induced conditions).

[0070] In the present invention, several plant promoters are provided which exhibit a high degree of constitutive and tissue-specific activity in trichomes, and which optionally respond substantially to one or more biotic and / or abiotic stresses Not sensitive. Such promoters are desirable for controlled expression of nucleic acid sequences in transgenic plants.

[0071] In one embodiment, the present invention provides the promoter region of a tomato gene (tomato terpene synthase and its orthologs and homologues) which confers higher trichome specificity in the host plant, Glandular trichome-specific express...

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Abstract

Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising suchtrichome-specific promoteror a chimeric or vector comprising such trichome-specific promoter. The invention further provides methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.

Description

technical field [0001] The present invention relates to trichome-specific plant promoters and uses thereof. The promoter can be used to express homologous or heterologous proteins in trichome cells, or to express active nucleic acid molecules, such as sense and / or antisense RNA. Nucleic acid sequences having promoter activity are provided, as well as chimeric genes, vectors and recombinant (transgenic) cells and organisms comprising them. Also provided are methods for making transgenic cells and organisms, especially plants and plant cells, comprising the promoter. This promoter is used for the production of various compounds in the trichomes, such as insecticides, volatile oils, fragrances or fragrances, pharmaceutical components, cytotoxic proteins, and the like. Background technique [0002] Trichomes are the various products of the epidermis in plants, including branched and unbranched hairs, vesicles, barbs, and stinging hairs. Trichomes are staked, multicellular pro...

Claims

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Application Information

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IPC IPC(8): C12N15/82
CPCC12N15/8223
Inventor 保罗·约翰·迪尔加德马里纳斯·威兰姆·普林斯马丁·德·福斯
Owner 凯金公司
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