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Plasmid extraction device

An extraction device and plasmid technology, applied in the field of experimental instruments, can solve the problem of time-consuming "plasmid DNA", and achieve the effect of saving operation time

Active Publication Date: 2015-07-29
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the time-consuming problem of extracting recombinant "plasmid DNA" in the prior art, the embodiment of the present invention provides a plasmid extraction device

Method used

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Experimental program
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Embodiment

[0026] The embodiment of the present invention provides a plasmid extraction device, such as figure 1 As shown, the plasmid extraction device includes: a first test tube 1 and a second test tube 2 arranged below the first test tube 1, the first test tube 1 includes: a first test tube 3, a hollow sleeve 4 and a shaft column 5, the second The top of a test tube 3 is provided with a first through hole 7 (see figure 2 ) and the injection hole 7a, one end of the sleeve 4 is located in the first test tube 3, and a stirring plate 6 is installed in the sleeve 4 located in the first test tube 3, and the other end of the sleeve 4 extends from the first through hole 7 Out of the first test tube 3, the bottom of the first test tube 3 is provided with a second through hole 8, and one end of the shaft column 5 passes through the inside of the sleeve 4 to connect with the plug 9, and the plug 9 is arranged in the second through hole 8, the size of the plug 9 matches the size of the second ...

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Abstract

The invention discloses a plasmid extraction device, and belongs to the field of experiment instrument. The plasmid extraction device comprises a first test tube and a second test tube arranged below the first test tube; the first test tube comprises a first test tube barrel, a hollow sleeve barrel, and a shaft column; the top part of the first test tube barrel is provided with a fist through hole and a filling hole; the second test tube comprises a filter tube, an adsorption tube, and a second test tube barrel; the filter tube is sleeved by the adsorption tube; and the adsorption tube is sleeved by the second test tube barrel. According to the plasmid extraction device, repeat delivering of samples into a centrifuge is avoided, operation time is shortened, and the whole extraction process can be completed in a few minutes.

Description

technical field [0001] The invention relates to the field of experimental instruments, in particular to a plasmid extraction device. Background technique [0002] A plasmid is a stable extrachromosomal genetic element. Plasmids can be artificially added with known or unknown DNA fragments in vitro to form recombined "plasmid DNA", and then this artificially synthesized new recombined "plasmid DNA" is injected into the bacteria and subsequently Bacteria multiply and reproduce in large numbers, and then collect this large number of bacteria, and use a special method to extract or separate the new recombinant "plasmid DNA" from the bacteria, so as to obtain a relatively large amount (several micrograms or several grams) biologically active novel recombinant "plasmid DNA". This recombined "plasmid DNA" can be used for various scientific experiments. [0003] Recombinant "plasmid DNA" is mostly extracted by kits, and the extraction process requires repeated centrifugation and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/24C12M1/12C12M1/02C12M1/00
CPCC12N15/101
Inventor 易初丽
Owner JIANGHAN UNIVERSITY
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