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Method for culturing double-micronucleus paramecium by inflating plastic film bag

A technology of plastic film bags and nuclear paramecia, applied in the field of practical tools and aquaculture, can solve the problems of low practicability, high technical requirements for operation, difficult to master, etc.

Inactive Publication Date: 2015-07-22
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paramecium separator has simple structure and convenient operation. It can separate paramecium from the paramecium solution mixed with various protozoa and algae, which is convenient for experimental application, especially for teaching experiments. Grasp, not practical

Method used

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  • Method for culturing double-micronucleus paramecium by inflating plastic film bag
  • Method for culturing double-micronucleus paramecium by inflating plastic film bag

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Embodiment Construction

[0016] A method for cultivating the paramecium dimicronuclei with inflation in a plastic film bag:

[0017] Step 1 Preparation of Paramecium Culture Solution

[0018] The proportions of the material composition by weight are as follows: 1 part of corn stalks, 3 parts of peanut shells, 4 parts of carrots, 2 parts of watermelon rind, 1 part of bran, 0.5 parts of pig blood, 2 parts of mushrooms, 0.8 parts of fungus, and earthworm powder 0.2 parts, 0.1 parts of silkworm chrysalis powder, 0.3 parts of milk powder, 3 parts of spinach, 2 parts of sheep manure, 1 part of chicken manure, 4 parts of cow manure, 1000 parts of boiled, cooled and then magnetized fresh water; During this period, stir and stir once every 6 hours, and finally remove the solid matter and discard it. The remaining liquid is allowed to stand and settle for 24 hours. The dregs are discarded. The supernatant is filtered with a 100-mesh sieve, and the dregs are discarded. For paramecium culture fluid;

[0019] St...

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Abstract

The invention discloses a method for culturing a double-micronucleus paramecium by inflating a plastic film bag, and belongs to the field of aquiculture. The technical key points are that a paramecium culture solution is prepared, then inflation culture is conducted in the plastic film bag, and two types of fresh water microalgae-chlamydomonas reinhardii and chlorella pyrenoidosa are complementally fed in the last four days of the culture. Compared with the traditional technology, the innovativeness is that the paramecium is cultured by adopting the plastic film bag, and the paramecium is quick in growth, strong in somatoplasm and high in yield; the plastic film bag is good in light-permeating property, can be repeatedly used, and has low possibility of being polluted, the operation is convenient, and fund can be saved.

Description

[0001] Technical field The present invention belongs to the field of aquaculture, and in particular relates to a method for inflating paramecium dimicronuclei with a plastic film bag. Background technique [0002] Paramecium belongs to the phylum Protozoa, Ciliae, Holothae, Hymenostomum, Pharynx, Parameciaceae, Paramecium genus, is a typical single-celled protozoa, likes to live in organic matter-rich and slightly acidic fresh water Among them, there are 4 species in total, namely, Paramecium paramecium, Paramecium dimicronucleate, Paramecium multinucleate, and Paramecium paramecium. The optimum growth temperature of Paramecium is 25°C, and the optimum reproduction temperature is 24-30°C. pH value 5.0-10.0 grows well, and the optimum pH value is 7-8. Among them, Paramecium dimicronuclei is often used as a single-cell biological model in biology teaching because of its large individuality, typical structure, fast reproduction, convenient observation, and easy collection and cu...

Claims

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Application Information

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IPC IPC(8): C12N1/10C12R1/90
CPCC12N1/10
Inventor 王培磊
Owner LINYI UNIVERSITY
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