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In-situ culturing device for deep-sea microbes

An in-situ culture, microorganism technology, applied in enzymology/microbiology devices, biochemical cleaning devices, biomass post-processing, etc., can solve the problem of inability to simulate, complex microbial living environment and natural conditions, and unable to completely simulate the natural survival of microorganisms. conditions and other issues, to achieve the effect of breaking through research bottlenecks, improving cultivability, and overcoming natural living conditions

Inactive Publication Date: 2015-07-22
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although they increase the number of microorganisms that can be cultivated to a certain extent, due to the complex living environment and natural conditions of microorganisms, such as: the diversity of microorganisms, chemical factors in the natural environment, and the relationship between biotic and abiotic factors in the environment interaction, as well as the balance of the global ecosystem at the microbial level, etc., so the above-mentioned cultivation methods cannot simulate or completely simulate the natural living conditions of microorganisms

Method used

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  • In-situ culturing device for deep-sea microbes
  • In-situ culturing device for deep-sea microbes
  • In-situ culturing device for deep-sea microbes

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Such as Figures 1 to 10 As shown, a deep-sea microbial in-situ culture device includes a sample culture box 1 and a culture box placement box 2. The sample culture box 1 includes a circular box body 11 and an open circular upper button cover 12 and lower button cover 13. The circular upper button cover 12 and the upper end surface of the box body 11 are each provided with an annular silicone sealing gasket 14, and a layer of filter membrane 15 is arranged between the two silicone sealing gaskets 14; the circular lower button cover 13 and the A ring-shaped silicone sealing gasket 14 is respectively provided on the lower end surface of the box body 11, and a layer of filter membrane 15 is arranged between the two silicone sealing gaskets 14; Membrane 15 and gasket 14 are fastened on the upper and lower ends of box body 11; like this, a cavity is formed by upper and lower filter membranes 15 and circular box body 11, agar is housed in the cavity For solid medium, the fil...

Embodiment 2

[0034] Such as Figures 1 to 10 As shown, the culture box placement box 2 in the first embodiment does not install the first hanging ring 23; after the sample culture box 1 is loaded into the culture box placement box 2, multiple culture box placement boxes 2 are then loaded into a placement box In the protective cover 3; the box protective cover 3 is a cuboid shape, and several second through holes 31 are provided on its four walls, the upper wall and the lower wall, for seawater to enter and exit at will; the upper wall of the box protective cover 3 can be opened Closed, used for loading and unloading the culture box placement box 2; the top surface of the upper wall of the placement box protective cover 3 is provided with a second hanging ring 32, which is used to hang the placement box protective cover 3 on the diving equipment ; see Figure 7 , 8 .

[0035] Put the storage box protective cover 3 loaded with multiple culture box storage boxes 2 in the deep sea, the seaw...

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Abstract

The invention discloses an in-situ culturing device for deep-sea microbes. The device comprises sample culturing boxes, culturing box placement cases and a placement case protection cover, wherein each sample culturing box comprises a box body, an upper locking cover and a lower locking cover; a sealing gasket, a filter membrane, a sealing gasket, the box body, a sealing gasket, a filter membrane and a sealing gasket are sequentially arranged between each upper locking cover and the corresponding lower locking cover; more than one sample culturing box can be accommodated in each culturing box placement case; more than one culturing box placement case can be accommodated in the placement case protection cover. The device has the advantages that the device can be directly arranged in deep sea, the natural environment of the deep-sea microbes can be maximally restored, and the deep-sea microbes can naturally grow; chemical substances can freely penetrate through the filter membranes, so that interaction between microbial communities can be ensured, and the culturability of the microbes is improved; the shortcoming of incapability of the conventional microbe culturing technology in simulating or completely simulating the natural conditions of existence for marine microbes is overcome, and the bottleneck in researches on nonculturable microbes is broken.

Description

technical field [0001] The invention belongs to the technical field of marine microorganism cultivation, and in particular relates to a device for in-situ cultivation of deep sea microorganisms. Background technique [0002] In recent years, due to the development of deep-sea exploration technology, the deep-sea world has gradually unveiled its mystery. Deep-sea microorganisms have formed unique genetic metabolism mechanisms and chemical defense mechanisms during the evolution process of adapting to complex environments (generally high pressure, low temperature, darkness, high salinity, oligonutrients, etc.), and are an important source of new drug active lead compounds. Although marine microbial culture technology has been developed for decades, studies have shown that the vast majority of deep-sea microorganisms cannot be cultured by current culture technology, and these microorganisms are called "unculturable microorganisms". "Uncultivable microorganisms" is a huge micro...

Claims

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Application Information

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IPC IPC(8): C12M1/00
Inventor 陈颢樊亚琴杜宁吴谡琦张学雷姜亚斌于楷本
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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