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A kind of Eucheuma polypeptide extract and its preparation method and application

The technology of kylin cabbage and its extract is applied in the field of kylin cabbage polypeptide extract and its preparation, which can solve the problem that the peptide components of kylin cabbage are not found to be used as antithrombotic drugs or health care products, and achieve good anti-platelet aggregation and inhibition of thrombus. The effect of forming, prolonging bleeding time and clotting time

Active Publication Date: 2018-01-09
海南和肽海洋资源开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no literature reports on the antithrombotic function of Eucheuma polypeptide components, and there are no reports of using Eucheuma polypeptide components as antithrombotic drugs or health care products at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of Eucheuma Polypeptide Extract

[0029] (1) Weigh 20 g of Eucheuma and grind it mechanically, and homogenize it with 160 mL of 25 mmol / L HCl solution to obtain a crude liquid.

[0030] (2) The crude liquid is relatively viscous, add an appropriate amount of saturated potassium chloride solution (at 20°C, the mass concentration of saturated potassium chloride solution is about 25%) to the crude liquid, so that the concentration of potassium chloride is 1.3%.

[0031] (3) Centrifuge the crude solution in step (2) (8000 rpm, centrifuge for 18 min), collect the supernatant, filter the supernatant with three layers of gauze, and use Sephadex G-50 gel column For chromatographic separation, the column height of the gel column is 28 cm, the inner diameter width is 1.8 cm, and the sample loading height is 5% of the column height of the gel column. After equilibration, the HCl solution with a concentration of 25 mmol / L was used for elution, and the flow r...

Embodiment 2

[0034] Example 2 Preparation of Eucheuma Polypeptide Extract

[0035] (1) Weigh 200 g of Eucheuma and grind it mechanically, and homogenize it with 2200 mL of 15 mmol / L HCl solution to obtain a crude liquid.

[0036] (2) The crude liquid is relatively viscous, add an appropriate amount of saturated potassium chloride solution to the crude liquid so that the concentration of potassium chloride is 1.8%.

[0037] (3) Centrifuge the crude liquid from step (2) (12000 rpm, centrifuge for 13 min), collect the supernatant, filter the supernatant with three layers of gauze, and use Sephadex G-50 gel column For chromatographic separation, the column height of the gel column is 32 cm, the inner diameter width is 2.2 cm, and the sample loading height is 7% of the column height of the gel column. After equilibration, the HCl solution with a concentration of 15 mmol / L was used for elution, and the flow rate of the eluent was controlled at 1.2 mL / min to obtain two sets of elution peaks.

...

Embodiment 3

[0040] Example 3 Preparation of Eucheuma Polypeptide Extract

[0041] (1) Weigh 1000g of Eucheuma and mechanically pulverize it, and homogenize it with 10 L of 20 mmol / L HCl solution to obtain a crude liquid.

[0042] (2) The crude liquid is relatively viscous, add an appropriate amount of saturated potassium chloride solution to the crude liquid so that the concentration of potassium chloride is 1.5%.

[0043] (3) Centrifuge the crude liquid from step (2) (10,000 rpm, centrifuge for 15 min), collect the supernatant, filter the supernatant with three layers of gauze, and use Sephadex G-50 gel column For chromatographic separation, the column height of the gel column is 30 cm, the inner diameter width is 2.0 cm, and the sample loading height is 10% of the column height of the gel column. After equilibration, the HCl solution with a concentration of 20 mmol / L was used for elution, and the flow rate of the eluent was controlled at 1.0 mL / min to obtain two sets of elution peaks. ...

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Abstract

The invention discloses an Eucheuma polypeptide extract and its preparation method and application. The present invention prepares a polypeptide extract from Eucheuma raw material. Experiments prove that the extract has good anti-platelet aggregation, prolongs bleeding time and Coagulation time, the new performance of inhibiting thrombus formation, the extract can be applied to the preparation of anti-platelet aggregation or anti-thrombotic drugs, drug components, drug precursors or health products.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an Eucheuma polypeptide extract and a preparation method and application thereof. Background technique [0002] Eucheuma (Eucheuma), also known as chicken foot vegetable, chicken jelly vegetable, belongs to algae. The algae are thick and fleshy, cylindrical, flat or flat, radiating or branched on both sides. There are about 20 species in this genus, and there are about 5 species in China. , is a tropical seaweed with high medicinal value. Eucheuma polypeptide components come from Eucheuma. [0003] Thrombotic disease is a kind of disease that seriously affects health, often manifested as myocardial infarction, ischemic cerebral infarction, and venous thromboembolism. At present, the clinical drugs for the treatment of thrombotic diseases are mainly divided into antiplatelet drugs, anticoagulant drugs and thrombolytic drugs. Antiplatelet drugs can inhibit the adhesion, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/18A61K38/00A61K36/02A61P7/02A23L33/00
Inventor 张海涛林向阳刘彬伍俊
Owner 海南和肽海洋资源开发有限公司
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