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Application of POU5F1B in diagnosis, treatment, prognosis and relapse prediction of tumor

A tumor and prognosis technology, used in gene therapy, anti-tumor drugs, medical preparations containing active ingredients, etc., can solve the problems of limited and immature markers of esophageal cancer.

Active Publication Date: 2015-07-08
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, global markers for predicting early recurrence and poor prognosis in esophageal cancer are immature, and tools to understand the risk of disease progression and guide the course of treatment for newly diagnosed esophageal cancer patients are limited

Method used

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  • Application of POU5F1B in diagnosis, treatment, prognosis and relapse prediction of tumor
  • Application of POU5F1B in diagnosis, treatment, prognosis and relapse prediction of tumor
  • Application of POU5F1B in diagnosis, treatment, prognosis and relapse prediction of tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: 8q24.21 is a hotspot of amplification in tumor genomes

[0061] 1. Tumorscape database analysis

[0062] Methods: Genomic copy number variation data in 3138 tumor samples (2526 tumor tissues mentioned later plus 611 tumor cell lines), 2526 tumor tissues and 611 tumor cell lines in the Tumorscape database were analyzed. The percentage of samples with increased copy number of a certain chromosome segment in 3138 tumor samples, 2526 tumor tissues and 611 tumor cell lines was presented in the form of circles using circos software, the outermost circle is the chromosome genome, different Chromosomes are shown in different colors, and each row in each chromosome represents a locus. The three circles inside represent the percentages of samples with increased copy number of a certain chromosome segment in 3138 tumor samples, 2526 tumor tissues and 611 tumor cell lines from outside to inside respectively. The degree of copy number amplification can be divided in...

Embodiment 2

[0077] Example 2: POU5F1B is located at the amplification peak of 8q24.21, and it is related to the prognosis of tumor patients

[0078] 1. Distribution of genes on chromosome 8

[0079] Methods: The previous analysis results suggested that a certain gene in the 8q24.21 segment could be used as a target for auxiliary diagnosis of tumors, so we searched which genes were distributed on the 8q24.21 segment.

[0080] Results: There were FAM84B, PCAT1, POU5F1B, MYC, PVT1, GSDMC, FAM49B and ASAP1 on the 8q24.21 segment.

[0081] 2. The results of TCGA database analysis showed that POU5F1B was most amplified in the 8q24.21 segment

[0082] method: Figure 6 In essence image 3 a part of.

[0083] Result: if Figure 6 As shown, POU5F1B is the gene with the highest degree of amplification in the 8q24.21 segment, and the amplification frequency of POU5F1B in 5443 cases of primary tumor tissues, 393 cases of metastatic tissues and 63 cases of recurrent tissues in the TGCA data...

Embodiment 3

[0151] Example 3: The amplification of POU5F1B promotes the expression of POU5F1B mRNA and enhances the characteristics related to tumor stem cells

[0152] 1. TCGA database analysis results show that the amplification of POU5F1B promotes the upregulation of POU5F1B mRNA expression

[0153] In order to explore the role of POU5F1B in tumors, the correlation between CNV (copy number variants) and RNA levels was analyzed.

[0154] Methods: The pan-cancer data in TCGA were classified according to the degree of POU5F1B amplification in the pan-cancer, including diploid (4415 cases), Gain (2857 cases) and Amplification (1014 cases).

[0155] All statistical analyzes were processed with SPSS17.0 statistical software. The independent t-test (constant variable) was used for comparison between different groups, the t-test was used for the comparison of means between two groups, and the χ test was used for the comparison of categorical variables 2 One-way analysis of variance was u...

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Abstract

The invention discloses an application of POU5F1B in diagnosis, treatment, prognosis and relapse prediction of tumor. In the invention, it is found that the copy number and the expression level of the gene POU5F1B in various tumors are closely related to transfer, relapse, patient prognosis and dryness of the tumor cells. A research result proves that by means of high expression of the POU5F1B, formation of TIC is promoted and further the in-vivo tumorigenic capability of esophagus cancer cells is enhanced, thereby reducing the survival rate, and oppositely, by means of inhibition of the expression of the POU5F1B, the formation of the TIC is promoted and further the in-vivo tumorigenic capability of the esophagus cancer cells is reduced, thereby increasing the survival rate, so that it is obviously that by means of inhibition of the endogenous POU5F1B, the in-vivo tumorigenic capability of the esophagus cancer cells can be significantly reduced, thereby increasing the survival rate of patients, which proves that the POU5F1B can be used as a marker for diagnosis, treatment, prognosis and relapse prediction. An inhibitor of the POU5F1B has a great clinical application value in preparation of a drug composition for tumor, so that the invention provides a new drug and a new method for effective treatment of tumor.

Description

technical field [0001] The present invention relates to the application of POU5F1B in tumor diagnosis, treatment, prognosis and recurrence prediction. Background technique [0002] Esophageal cancer is one of the common malignant tumors, its incidence rate ranks eighth among malignant tumors, and its mortality rate ranks sixth. The incidence of esophageal cancer in my country is extremely high, accounting for about 50% of the world, and my country has become the country with the highest mortality rate of esophageal cancer in the world. [0003] At present, the treatment methods for esophageal cancer mainly include surgery, radiotherapy and chemotherapy, but the prognosis of patients is still poor. 75% of patients die within 1 year after receiving treatment, and only 5-10% of patients survive. Time can reach 5 years. [0004] The discovery and rational application of tumor markers are the prerequisites for the prediction of tumor recurrence. To date, global markers for pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K48/00A61K31/7088A61P35/00
CPCA61K48/00C12Q1/6886C12Q2600/158
Inventor 宋立兵李隽张鑫谭展瑶吴淑
Owner SUN YAT SEN UNIV CANCER CENT
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