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Application of Compound Amino Acids in Preparation of Drugs for Improving Bacteria's Sensitivity to Antibiotics

A compound amino acid and antibiotic technology, applied in the field of biomedicine, can solve the problems of unsatisfactory bacterial effect and limited effect, and achieve the effect of reducing bacterial survival rate, less adverse reactions, and improving bactericidal effect.

Active Publication Date: 2021-08-13
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the limited effect of a single amino acid combined with antibiotics to improve the sensitivity of bacteria to antibiotics, especially the unsatisfactory effect on bacteria that have produced drug resistance. Protocols for Antibiotic Sensitivity

Method used

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  • Application of Compound Amino Acids in Preparation of Drugs for Improving Bacteria's Sensitivity to Antibiotics
  • Application of Compound Amino Acids in Preparation of Drugs for Improving Bacteria's Sensitivity to Antibiotics
  • Application of Compound Amino Acids in Preparation of Drugs for Improving Bacteria's Sensitivity to Antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 compound amino acid improves the sensitivity of cefazolin Escherichia coli drug-resistant bacteria to cefazolin

[0046] 1.1 Artificial passage of drug-resistant bacteria-cefazolin Escherichia coli-resistant bacteria

[0047]Using Escherichia coli K12 as the starting strain, it was continuously passaged in cefazolin liquid medium containing 1 / 2 minimum inhibitory concentration (MIC), and streaked on the plate every five generations to obtain a single clone and determine its To the MIC of cefazolin; until the MIC of obtaining bacterial strain is 80 μ g / mL, promptly is 64 times of initial bacterial strain (see specifically figure 1 ), named as cefazolin-resistant Escherichia coli (K12-R CZO ).

[0048] 1.2 The compound amino acid improves the sensitivity of Escherichia coli sensitive bacteria and cefazolin Escherichia coli resistant bacteria to cefazolin

[0049] Experimental materials: three compound amino acids with total amino acid contents of 5%, 8.5%,...

Embodiment 2

[0076] Example 2 compound amino acid improves the sensitivity of bacteria to antibiotics and has antibiotic specificity

[0077] 2.1 Significantly improve the bactericidal efficiency of cephalosporin antibiotics

[0078] Further study after adding 0.125, 0.5, 2.0, 8.0, 32, 64mM compound amino acids, cephalosporin antibiotics (10μg / mL ceftriaxone, 200μg / mL ceftiofur) after 6h of Escherichia coli sensitive bacteria K12, the bacterial survival rate effect; the results can be found in Figure 8 .

[0079] It can be seen from the figure that after 0.125, 0.5, 2.0, 8.0, 32, 64mM compound amino acid combined with ceftriaxone, the bacterial survival rate was 73.1%, 54.3%, 13.5%, 0.115%, 0.00827%, 0.00345% and 0.00173%, K12 The survival rate was reduced by 1.35 times to 42352 times; after cooperating with ceftiofur, the bacterial survival rate was 61.9%, 77.7%, 20.7%, 0.00907%, 0.00589%, 0.00213% and 0.000711%, and the survival rate of sensitive bacteria K12 was reduced by several ti...

Embodiment 3

[0089] The influence of embodiment 3 compound amino acid synergistic cefazolin on the survival rate of other types of bacteria

[0090] Select other Gram-negative bacteria such as Vibrio alginolyticus, Vibrio parahaemolyticus, and Pseudomonas aeruginosa, as well as the Gram-positive bacteria Bacillus subtilis, to investigate the bactericidal effect of compound amino acids and cefazolin on them; Among them, the compound amino acid concentration is 2mM, cefazolin is 80μg / mL, incubated for 6h, and the relative survival rate of bacteria is calculated; the results can be found in Figure 12 .

[0091] As can be seen from the figure, after 2mM compound amino acid is added, cefazolin causes the survival rate of Vibrio alginolyticus to reduce from 62.1% to 13.9%, and the synergistic multiple reaches 4.5 times ( Figure 12 A); Cause the survival rate of vibrio parahaemolyticus to reduce to 1.54% from 79.3%, synergy multiple reaches 51.6 times ( Figure 12 B); Cause the survival rate ...

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Abstract

The invention belongs to the technical field of biomedicine, and in particular relates to the application of compound amino acids in the preparation of drugs for improving the sensitivity of bacteria to antibiotics. Experiments have proved that the combination of compound amino acids and antibiotics can increase the sensitivity of bacteria to antibiotics, and promote the entry of antibiotics into bacterial cells, significantly increase the content of antibiotics in cells, improve the bactericidal effect of antibiotics, thereby reducing the survival rate of bacteria and treating bacteria Diseases caused by infestation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of compound amino acids in the preparation of drugs for improving the sensitivity of bacteria to antibiotics. Background technique [0002] In the early twentieth century, the discovery and application of antibiotics brought bacterial infections under control. However, with the emergence of drug-resistant bacteria and superbugs, the effectiveness of antibiotics in treating bacterial diseases has been reduced or even ineffective, and human beings are facing new challenges. At present, the speed of bacteria developing drug resistance is gradually accelerating, and the difficulty and time of new drug development are gradually increasing. If this continues, there may be a dangerous situation where there will be no available drugs in the near future, seriously threatening human health and life safety. [0003] Amino acids, as small molecular compo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/06A61K31/198A61K31/4172A61K31/405A61K31/401A61K31/546A61K31/43A61K31/407A61K31/4709A61K31/7036A61P31/04
CPCA61K45/06A61K31/198A61K31/4172A61K31/405A61K31/401A61K31/546A61K31/43A61K31/407A61K31/4709A61K31/7036A61P31/04A61K2300/00Y02A50/30
Inventor 李惠彭博彭宣宪赖诗诗
Owner SUN YAT SEN UNIV
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