A pig-derived Geotrichum candidum producing strain and the liquid feed attractant prepared therefrom
A technology of liquid feed and food attractant, applied in the direction of animal feed, animal feed, fungi, etc., can solve the problems of limited development and application of natural flavors, long growth cycle of raw materials, and limited quantity.
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[0028]The method for isolating and screening a Geotrichum fructiferica strain YSJY-25 from fresh feces of healthy sows is as follows. Take fresh sow manure and put it into a sterile container, seal it, store it in an ice box, and quickly send it to the laboratory for routine strain isolation. The separation plate medium is PDA medium. After gradient dilution, incubate the coated plate upside down at 30°C for 2-3 days, pick several single colonies of Geotrichum candidum on the PDA slant medium, and culture upside down at 30°C for 2-3 days ,spare.
[0029] The above-mentioned slant strains were respectively inserted into a loop of PDA liquid medium in a 25ml / 250ml bottle, cultivated on a shaker at 30°C at 180rpm for 16h, centrifuged to get the supernatant, and measured the protease activity. The strain with the highest protease activity was used as the experimental strain. Named YSJY-25.
[0030] Observation and description of plate colony: colony is white, flat, round, with n...
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