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A pig-derived Geotrichum candidum producing strain and the liquid feed attractant prepared therefrom

A technology of liquid feed and food attractant, applied in the direction of animal feed, animal feed, fungi, etc., can solve the problems of limited development and application of natural flavors, long growth cycle of raw materials, and limited quantity.

Inactive Publication Date: 2018-06-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long growth cycle and limited quantity of natural animal and plant flavors, the development and application of natural flavors are limited.

Method used

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  • A pig-derived Geotrichum candidum producing strain and the liquid feed attractant prepared therefrom
  • A pig-derived Geotrichum candidum producing strain and the liquid feed attractant prepared therefrom
  • A pig-derived Geotrichum candidum producing strain and the liquid feed attractant prepared therefrom

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Embodiment Construction

[0028]The method for isolating and screening a Geotrichum fructiferica strain YSJY-25 from fresh feces of healthy sows is as follows. Take fresh sow manure and put it into a sterile container, seal it, store it in an ice box, and quickly send it to the laboratory for routine strain isolation. The separation plate medium is PDA medium. After gradient dilution, incubate the coated plate upside down at 30°C for 2-3 days, pick several single colonies of Geotrichum candidum on the PDA slant medium, and culture upside down at 30°C for 2-3 days ,spare.

[0029] The above-mentioned slant strains were respectively inserted into a loop of PDA liquid medium in a 25ml / 250ml bottle, cultivated on a shaker at 30°C at 180rpm for 16h, centrifuged to get the supernatant, and measured the protease activity. The strain with the highest protease activity was used as the experimental strain. Named YSJY-25.

[0030] Observation and description of plate colony: colony is white, flat, round, with n...

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Abstract

The present invention isolates and screens the Geotrichum fruticosa strain obtained from the fresh feces of healthy sows, the strain name is YSJY‑25, and it is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC No.9061. The aroma-producing liquid food attractant is prepared by liquid fermentation of the strain, and the liquid feed food attractant can produce ethyl isobutyrate, ethyl propionate, isoamyl acetate, ethyl n-hexanoate, and isobutyl acetate as detected by a gas spectrometer. Esters, ethyl 2‑methylbutyrate and other fruity substances. The bacterial strain obtained by the invention has the specificity of the pig-derived host, and the prepared liquid feed attractant has simple process and low cost, and is suitable as the pig-derived feed attractant and feed additive.

Description

technical field [0001] The invention relates to a pig-derived Geotrichum candidum strain and a liquid feed attractant prepared by using the strain. Background technique [0002] In my country's GB10647-89 standard, additives added to feed that can improve feed palatability, increase animal appetite and feed intake are called feed attractants, also known as feed flavoring agents, flavoring agents, and appetite enhancers. Dr. D.V.Catron of Iowa State University in the United States was the first to start using feed attractants. They added a substance that can improve the palatability of feed to pig and chicken feed, and the effect was remarkable. In 1946, the United States established the Feed Flavor Company (FCA). In 1963, Japan introduced feed flavor from FCA and started independent research and development from piglet flavor. In the 1980s, Europe and the United States had widely used feed attractants. Agents expanded to countries in Eastern Europe, CIS and Southeast Asia. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23K10/18C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 徐速江连洲穆莹孙慧齐宝坤邵红
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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