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Swine-derived Geotrichum candidum strain capable of producing fruity flavor and liquid feed attractant prepared from swine-derived Geotrichum candidum strain capable of producing fruity flavor

A technology of Geotrichum candidum and liquid feed, applied in animal feed, animal feed, fungi, etc., can solve the problems of limited quantity, limited development and application of natural spices, long growth cycle of raw materials, etc.

Inactive Publication Date: 2015-07-08
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the long growth cycle and limited quantity of natural animal and plant flavors, the development and application of natural flavors are limited.

Method used

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  • Swine-derived Geotrichum candidum strain capable of producing fruity flavor and liquid feed attractant prepared from swine-derived Geotrichum candidum strain capable of producing fruity flavor
  • Swine-derived Geotrichum candidum strain capable of producing fruity flavor and liquid feed attractant prepared from swine-derived Geotrichum candidum strain capable of producing fruity flavor
  • Swine-derived Geotrichum candidum strain capable of producing fruity flavor and liquid feed attractant prepared from swine-derived Geotrichum candidum strain capable of producing fruity flavor

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Embodiment Construction

[0028] The method for isolating and screening a Geotrichum fructiferica strain YSJY-25 from fresh feces of healthy sows is as follows. Take fresh sow manure and put it into a sterile container, seal it, store it in an ice box, and quickly send it to the laboratory for routine strain isolation. The separation plate medium is PDA medium. After gradient dilution, incubate the coated plate upside down at 30°C for 2-3 days, pick several single colonies of Geotrichum candidum on the PDA slant medium, and culture upside down at 30°C for 2-3 days ,spare.

[0029] The above-mentioned slant strains were respectively inserted into a loop of PDA liquid medium in a 25ml / 250ml bottle, cultivated on a shaker at 30°C at 180rpm for 16h, centrifuged to get the supernatant, and measured the protease activity. The strain with the highest protease activity was used as the experimental strain. Named YSJY-25.

[0030] Observation and description of plate colony: colony is white, flat, ...

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Abstract

The invention provides a swine-derived fruity flavor-producing Geotrichum candidum strain isolated and screened from fresh excrements of healthy sows. The strain is named as YSJY-25 and preserved in China General Microbiological Culture Collection Center with an accession number of CGMCC No. 9061. According to the invention, the strain is used to prepare an aroma-producing liquid feed attractant through liquid fermentation. According to detection results of a gas chromatography-mass spectrometer, the liquid feed attractant is able to produce fruity matters like ethyl isobutyrate, ethyl propionate, isoamyl acetate, ethyl caproate, isobutyl acetate and ethyl 2-methylbutyrate. The strain obtained in the invention has swine-derived host specificity; meanwhile, the prepared liquid feed attractant is simple to prepare, has low cost and is applicable as a swine-derived feed attractant and a feed additive.

Description

technical field [0001] The invention relates to a pig-derived Geotrichum candidum strain and a liquid feed attractant prepared by using the strain. Background technique [0002] In my country's GB10647-89 standard, additives added to feed that can improve feed palatability, increase animal appetite and feed intake are called feed attractants, also known as feed flavoring agents, flavoring agents, and appetite enhancers. Dr. D.V.Catron of Iowa State University in the United States was the first to start using feed attractants. They added a substance that can improve the palatability of feed to pig and chicken feed, and the effect was remarkable. In 1946, the United States established the Feed Flavor Company (FCA). In 1963, Japan introduced feed flavor from FCA and started independent research and development from piglet flavor. In the 1980s, Europe and the United States had widely used feed attractants. Agents expanded to countries in Eastern Europe, CIS and Southeast Asia. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K1/16C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 徐速江连洲穆莹孙慧齐宝坤邵红
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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