Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nitzschia closterium culture medium

A technology of Nitzschia and culture medium, applied in the direction of single-cell algae, microorganism-based methods, microorganisms, etc., can solve the problems of low biomass, unfavorable large-scale cultivation, slow growth, etc., and achieve high cell density and suitable for large batches Large-scale culture production, the effect of increasing the concentration of algae cells

Inactive Publication Date: 2015-07-08
王兆伟
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main problems in the large-scale cultivation of Nitzschia minor are slow growth and low biomass, which are not conducive to large-scale cultivation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nitzschia closterium culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] (1) Use natural seawater to configure the following formula of Nitzschia microcystis culture medium: NaNO 3 80mg / L, NaH 2 PO 4 8mg / L, ferric ammonium citrate 2.5mg / L, Na 2 EDTA12mg / L, Na 2 SiO 3 9H 2 O20mg / L, diphenylurea 0.1mg / L, p-chlorophenoxyacetic acid 0.1mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O23μg / L, MnCl 2 4H 2 O178μg / L, CuSO 4 ·5H 2 O10μg / L, Na 2 MoO 4 2H 2 O7.3μg / L and CoCl 2 ·6H 2 O12μg / L.

[0011] (2) Inoculate Nitzschia smalle in the logarithmic growth phase into the medium for cultivation, light for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at 20 degrees Celsius, and the cell concentration of Nitzschia lunster in the medium was recorded at regular intervals, see Table 1 for details.

Embodiment 2

[0013] (1) Use natural seawater to configure Nannochloropsis medium with the following formula: NaNO 3 120mg / L, NaH 2 PO 4 12mg / L, ferric ammonium citrate 3.5mg / L, Na 2 EDTA12mg / L, Na 2 SiO 3 9H 2 O30mg / L, diphenylurea 1mg / L, p-chlorophenoxyacetic acid 1mg / LVB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O23μg / L, MnCl 2 4H 2 O178μg / L, CuSO 4 ·5H 2 O10μg / L, Na 2 MoO 4 2H 2 O7.3μg / L and CoCl 2 ·6H 2 O12μg / L.

[0014] (2) Inoculate Nitzschia smalle in the logarithmic growth phase into the medium for cultivation, light for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at 20 degrees Celsius, and the cell concentration of Nitzschia lunster in the medium was recorded at regular intervals, see Table 1 for details.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a nitzschia closterium culture medium comprising the following components, by mass concentration: 80-120mg / L of NaNO3, 8-12mg / L of NaH2PO4, 2.5-3.5mg / L of ferric ammonium citrate, 10-12mg / L of Na2EDTA, 20-30mg / L of Na2SiO3.9H2O, 0.005-0.007mg / L of VB1, 0.04-0.06mg / L of VB12, 22-24mu g / L of ZnSO4.4H2O, 175-180mu g / L of MnCl2.4H2O, 9-11mu g / L of CuSO4.5H2O, 7.0-7.5mu g / L of Na2MoO4.2H2O, 11-13mu g / L of CoCl2.6H2O, and a plant growth regulator comprising 0.1-1mg / L of diphenyl urea and 0.1-1mg / L of p-chlorophenoxyacetic acid. When the culture medium is used for culturing nitzschia closterium, advantages of fast growth speed and high cell density are shown. The culture medium is suitable for large-scale productions.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a culture medium for Nitzkiris small. Background technique [0002] Small Crescent Nitzophrenia belongs to Diatomphyta, Plumenaea, Dirhomboides, Nitzphyceae, Nitzphylla, the center of the cell expands into a spindle shape, and the two ends are acuminate, like a crescent. The cells are 12-23 μm long and 2-3 μm wide, with a nucleus in the center. Chromosome yellowish brown, two pieces, located on both sides of nucleus. Distributed in seawater, brackish and freshwater waters of the world, it occurs in every sea area of ​​my country. The optimum salinity range is 25-32%, the optimum temperature is 15-20°C, and the growth will stop if the water temperature exceeds 28°C; the optimum light intensity is 3000-8000lx, avoid direct sunlight during cultivation; the optimum pH is 7-10, and the optimum is 7.5-8.5 . Small crescent algae, commonly known as "small diatoms", is rich in protein, carbo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟
Owner 王兆伟
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com