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Intermediate infrared spectrometer

An infrared spectrometer and infrared detection technology, applied in the measurement of color/spectral characteristics, etc., can solve problems such as poor stability, large spatial jitter, and poor spot quality, and achieve the effects of system stability, convenient operation, and high-speed data collection.

Active Publication Date: 2015-07-01
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

This method needs to go through a nonlinear process of Raman frequency shift, resulting in the generated 1900nm laser, the spot quality is relatively poor, and the spatial jitter is very large, and the long-term working stability of the system is also poor.

Method used

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Embodiment Construction

[0027] Such as figure 1 As shown, the mid-infrared spectrometer provided by the present invention that can achieve nanosecond time resolution by pulse heating mainly includes a broadband tunable carbon monoxide laser 10, a nanosecond short pulse Q-switched holmium laser 20, a pump detection optical system and a data acquisition system 40. The mid-infrared spectrometer of the present invention can accurately measure the transient infrared spectrum data of protein dynamic structure changes and the dynamic information of protein dynamic structure changes, and the precision of its infrared spectrum data can reach 1×10 -4 OD.

[0028] The various components of the mid-infrared spectrometer are described in detail below:

[0029] The high-stability low-temperature flowing broadband tunable carbon monoxide laser 10 is used to emit mid-infrared detection laser light to the protein to be tested to detect the secondary structure information of the protein to be tested, which can realiz...

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Abstract

The invention discloses an intermediate infrared spectrometer for realizing nanosecond time resolution by using pulse warming. The intermediate infrared spectrometer is used for measuring dynamic structure change of protein, and comprises a wideband tunable carbon oxide laser for emitting intermediate infrared detection laser to a to-be-detected protein solution, a nanosecond Q-modulation holmium laser for emitting pumping laser to heat the to-be-detected protein solution, a pumping detection light path system for detecting the strength of the intermediate infrared detection laser penetrating through the to-be-detected protein solution, and a data acquiring system for acquiring the strength data detected by the pumping detection light path system. The intermediate infrared spectrometer disclosed by the invention can be used for accurately measuring instant infrared spectrometry data of protein dynamic structure change and dynamics information of the protein dynamic structure change, the high-precision measurement on the protein secondary protein structure dynamic structure can be realized, and the infrared spectrometry data precision can reach 1*10<-4>OD.

Description

technical field [0001] The invention relates to the field of ultrafast time-resolved mid-infrared spectrum measurement, in particular to a mid-infrared spectrometer. Background technique [0002] Protein science is the fastest growing and most popular science of human science in the 21st century. Infrared spectroscopy has been widely used in protein science research because it contains a large amount of material structure information. At present, there are two main fields of using infrared spectroscopy to study the structural changes of proteins: the steady-state structural changes of proteins and the dynamic structural changes of proteins. Infrared spectroscopy to study the steady-state structural changes of proteins mainly relies on Fourier transform infrared spectroscopy (FTIR). It can give the basic structural information of the protein, but cannot give the dynamic structural information of the protein. [0003] In order to study the dynamic structural changes of prot...

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Application Information

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IPC IPC(8): G01N21/39
Inventor 李得勇翁羽翔
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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