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Preservative composition

A technology for composition and preservative, applied in the field of compositions for preservatives, can solve the problems of carcinogenic risk, inability to detect, and affect the real results of detection, and achieve the effect of reducing carcinogenic risk and ensuring accuracy

Active Publication Date: 2015-06-10
广州维帝医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, with the development of immunocytochemistry and nucleic acid chemistry research, it has been found that these preservative systems contain free aldehydes, especially free formaldehyde, which has a strong cross-linking and destructive effect on proteins and nucleic acids, directly causing people When in contact with biological samples or product preparations with such preservatives, allergic reactions, toxic reactions, and even cancer risks
The use of such additives in the field of biological samples, due to the destruction of proteins and nucleic acids, directly leads to the inability to detect or seriously affect the real results of the detection of such substances, causing the test results to deviate from the real situation and unable to give scientific conclusions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: For processing blood samples

[0032] This is an example of the application of the preservative composition of the present invention to biological products.

[0033] When preparing a preservative for processing 10ml of blood samples, first calculate according to the volume of 300 microliters of the added preservative, according to the preferred chelation stabilizer EDTA-K 3 The concentration of the blood is 2.2mg / ml, and the concentration of the anticoagulant in the required additives is calculated to be 73mg / ml. If 100ml of preservative is configured, EDTA-K needs to be added 3 7.3g. Other additives are calculated according to the following standards, the concentration of cytoprotective agent is 0.005g / ml blood; the molar ratio of cytoprotective agent to inhibitor is 1:1.

[0034] The final preparation ratio of each added component of the 100ml preservative is as follows:

[0035] EDTA-K3 7.3g 5,5-Dimethylhydantoin 16.7g N-acetate fo...

Embodiment 2

[0038] Example 2: For the treatment of cosmetic liquid preparations

[0039] This is an example of the application of the preservative composition of the present invention to non-biological samples.

[0040] When preparing a preservative for processing 10ml liquid formulations, first add 300 microliters of preservative volume, according to the preferred chelating stabilizer EDTA-Na 2 .2H 2 The concentration of O is 2.2mg / ml for cosmetic liquid preparations, and the concentration of anticoagulant in the required preservative is calculated to be 73mg / ml. If 100ml of preservative is configured, EDTA-Na needs to be added 2 .2H 2 O 7.3g. Other additives are calculated according to the following standards, the concentration of the cell protection agent is 0.002g / ml cosmetic liquid preparation; the molar ratio of the cell protection agent to the inhibitor is 1:1.

[0041] Preferably, when preparing the finished product of 100ml preservative, the preparation ratio of each added in...

Embodiment 3

[0046] This embodiment is improved on the basis of Embodiment 1.

[0047] The preservative composition comprises:

[0048] a) one or more cytoprotective agents;

[0049] b) one or more inhibitors;

[0050] c) one or more chelating stabilizers;

[0051] d) one or more acid-base buffers;

[0052] e) one or more antioxidants;

[0053] f) one or more antibiotics against hemolytic streptococci;

[0054]Among them, the chelating stabilizer EDTA is added at 2.2 mg / ml liquid sample or product preparation, that is, 2.2 mg EDTA is added per milliliter of liquid sample or product preparation; The molar ratio of the agent is 1:1; the cytoprotective agent is 0.005g / ml liquid sample or preparation, that is, 0.005g of cytoprotective agent is added per milliliter of liquid sample or product preparation; the inhibitor is based on the molar ratio of cytoprotective agent and inhibitor Corresponding addition; the amount of acid-base buffer added is 3% of the volume fraction of the liquid sa...

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Abstract

The invention relates to a preservative composition. The preservative composition comprises the following substances: a) one or multiple cell protective agents; b) one or multiple inhibitors; c) one or multiple chelate stabilizers; and d) one or multiple acid-base buffer solutions, wherein the cell protective agent is one or more than one of allantoin, 5,5-dimethyl hydantoin and oxazolidine, the inhibitor is one or more than one of N-acetic acid methylenimine and N-(3-acetic acid-amylamine), the chelate stabilizer is one or more than one of ethylenediaminotetraacetate, oxalate, heparinate and citrate, and the acid-base buffer agent can be glycine-sodium hydroxide-hydrochloride buffer solution. The preservative composition provided by the invention can be used for preserving a product preparation or a biological sample for a long time at normal temperature, the preservative system does not contain free aldehydes, is safer and more reliable and is beneficial to maintaining of nucleic acid completeness in the product preparation and the biological sample, and aldehyde detection accuracy is guaranteed.

Description

technical field [0001] The present invention relates to a composition used for preservatives, especially a composition of preservatives without aldehyde substances used for preserving product preparations or biological samples. Background technique [0002] In order to prevent or delay the deterioration of a certain substance during long-term storage, a preservative is usually added to prevent the substance from deteriorating within a certain storage period. This preservative does not affect its detection or use. [0003] Typical examples of substances requiring preservatives include biological samples and various product preparations. In these fields, a class of preservatives more commonly used in the prior art is aldehyde preservatives, the main types of which include: [0004] (1) Aldehydes themselves, such as formaldehyde, acetaldehyde, glutaraldehyde, etc.; [0005] (2) Formaldehyde donors and formaldehyde derivatives, such as 5-bromo-5 nitro-1,3-dioxane, 2-bromo-2 ni...

Claims

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Application Information

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IPC IPC(8): A01N1/00A01N1/02A01N3/00
Inventor 布丽斯曾晨光周启明杨习锋
Owner 广州维帝医疗技术有限公司
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