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Composition containing extracts of cichorium intybus and poncirus trifoliata for strengthening skin barrier

A skin barrier and composition technology, which is applied in the field of skin barrier strengthening compositions, can solve problems such as bad skin effects, skin irritation, difficulty in manufacturing, etc., so as to increase moisture retention, strengthen skin barrier, prevent and improve atopy effect of dermatitis

Inactive Publication Date: 2015-06-03
RADIANT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are germ essence, pantethein, fatty acid esters, etc. as well-known substances having a sebum secretion promoting action, but it is difficult to manufacture them into dosage forms due to the nature of the substances
[0006] Although various substances are used to improve the skin barrier function, the use of chemical raw materials can actually cause skin irritation, allergies, etc., and actually have a bad effect on the skin. In order to solve such problems, it is urgent to develop natural ingredients from nature

Method used

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  • Composition containing extracts of cichorium intybus and poncirus trifoliata for strengthening skin barrier
  • Composition containing extracts of cichorium intybus and poncirus trifoliata for strengthening skin barrier
  • Composition containing extracts of cichorium intybus and poncirus trifoliata for strengthening skin barrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the manufacture of chicory (Cichorium intybus), citrus orange (Poncirus trifoliata RAFIN), chicory (Cichorium intybus) and citrus orange (Poncirus trifoliata RAFIN) mixed extract

[0041] 50 g of dried chicory (Cichorium intybus) (Daiko Pharmaceutical) and citrus (Poncirus trifoliata RAFIN) (Daiko Pharmaceutical) were made into powder using a pulverizer, and extracted with 1 L of 70% ethanol at room temperature 3 hours, and then filtered with Whatman NO.4 filter paper and Advantec NO.5C filter paper. After concentrating the filtrate with a vacuum rotary concentrator, the yield of chicory was 21.76%, and the yield of Hovenia was 14.06%. For the chicory + citrus mixture, the two natural powders were mixed at a ratio of 1:1, and the extract was made by the above method.

experiment example 1

[0042] Experimental Example 1: Cell Culture

[0043] [Sebocyte, SZ95]

[0044] Use added 10% fetal bovine serum (Fetal bovine serum (FBS)), 5ng / ml human epidermal growth factor (human epidermal growth factor), 1mM CaCl 2 Dulbecco's modified eagle medium (DMEM) / F12 (1:1) in CO 2 Incubator (37°C, 5% CO 2 ) in which SZ95 cells were cultured, and the medium was replaced every 2-3 days.

[0045] [Fibroblast, CCD-986SK]

[0046] Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) was used in CO 2 Incubator (37°C, 5% CO 2 CCD-986 or cells were cultured in ), and the medium was changed every 2-3 days.

experiment example 2

[0047] Experimental Example 2: Cytotoxicity Evaluation (MTT assay)

[0048] In order to evaluate the cytotoxicity of the mixture extracts of Cichorium intybus, Poncirus trifoliata RAFIN, Cichorium intybus and Poncirus trifoliata RAFIN produced in Example 1, MTT assay was carried out. confirmed.

[0049] SZ95 and CCD-986SK cells were separately planted into 96-well plates at a concentration of 1×105 cells / ml, and incubated at 37°C, 5% CO 2Cultured in an incubator for 24 hours. After the culture, all the medium was removed, and 100 ul of the sample diluted to an appropriate concentration (25 ug / ml to 200 ug / ml) in a medium not containing serum was treated in each well, and cultured for 24 hours. After culturing for 24 hours, the MTT reagent was dissolved in PBS at a concentration of 5 mg / ml, and 20 μl each was added to culture for 4 hours. Remove all the medium containing MTT reagents and samples, add 100ul of acid isopropanol to each well, and stir in a shaker for 10-15 minu...

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Abstract

The invention relates to a composition containing extracts of cichorium intybus and poncirus trifoliata for strengthening a skin barrier. In particular, compared with individual extracts of cichorium intybus (cichorium intybus) and poncirus trifoliate (poncirus trifoliate RAFIN), by virtue of the mixed extracts of the cichorium intybus and the poncirus trifoliate, cell growth of sebocyte is increased; the amount of lipid synthesized from the sebocyte is obviously increased; and the amount of hyaluronan as an important moisture factor in the skin is obviously increased. Therefore, the composition can be effectively used as a composition for strengthening skin barriers of infants with insufficient lipid biosynthesis capability and a composition for preventing and treating atopic dermatitis.

Description

technical field [0001] The present invention relates to a composition for strengthening the skin barrier, and a composition for preventing and treating atopic dermatitis. Background technique [0002] The skin is composed of the epidermis and the dermis. The outermost stratum corneum of the skin is a skin barrier that protects the living body from physical and chemical stimuli and microorganisms in the external environment, and properly regulates the evaporation of water to maintain the homeostasis of the living body. . In order to maintain a healthy skin state, the stratum corneum needs to contain a certain amount of water, and the corneocytes need to form a balance with lipid components, amino acids, urea, organic acids and other natural moisturizing factors and sebum, and if the balance of such elements is broken or lacking , the moisture content of the stratum corneum of the skin decreases, and the skin barrier function is impaired, resulting in dry skin. [0003] Chil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/75A61P17/00A61K8/97A61Q19/00
Inventor 崔信旭洪仁基裴炯珍朴瀞殷金恩智张喜庭
Owner RADIANT
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