Gout serum miRNAs biomarkers and method for detecting expression quantity thereof

A biomarker and expression technology, applied in the field of biotechnology and medicine, to achieve the effect of great theoretical significance

Active Publication Date: 2015-05-27
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

miRNAs can exist stably in the blood for a long time, and various treatment methods such as repeated freezing and thawing and long-term storage will not cause the loss of serum miRNAs. However, the acquisition of peripheral blood samples is very simple, and gout-specific miRNAs can be detected in blood and can be detected. It is of great significance to use it as a biological marker of gout, and there are no public reports about gout serum miRNAs

Method used

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  • Gout serum miRNAs biomarkers and method for detecting expression quantity thereof
  • Gout serum miRNAs biomarkers and method for detecting expression quantity thereof
  • Gout serum miRNAs biomarkers and method for detecting expression quantity thereof

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Embodiment Construction

[0019] The present invention will be described in detail below by means of the embodiments in conjunction with the accompanying drawings. The conditions and methods not indicated in the embodiments are all carried out according to conventional conditions.

[0020] In this embodiment, gout serum miRNAs biomarkers are used to detect gout, and the specific process is as follows:

[0021] 1. Use Microarray chips to screen candidate gout-related miRNAs:

[0022] Collect the serum of 4 cases of acute gouty arthritis, 4 cases of simple hyperuricemia and 4 cases of healthy controls, use a coagulation tube to collect whole blood, separate the serum within two hours after blood collection, and gently invert the blood collection tube to mix 4- 5 times, stand upright at room temperature until the blood is completely coagulated, centrifuge at 1000g for 10 minutes, and separate the serum; transfer the separated serum to a separate cryopreservation tube, and pack it according to 500ul / tube; ...

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Abstract

The invention belongs to the fields of biotechnology and medicine, and relates to gout serum miRNAs biomarkers and a method for detecting the expression quantity thereof. The gout serum miRNAs biomarkers comprise hsa-miR-3146, hsa-miR-4449, hsa-miR-4531, hsa-miR-451a, hsa-miR-223-3p, hsa-miR-589-5p and hsa-miR-885-5p. These markers serving as important biological detection indexes of serum can be used for preparing tools such as gout diagnosis reagents or bio-chips and the like, have important value in clinical early accurate diagnosis of gout, provide theoretical basis for molecular level diagnosis of gout and research of gout serum miRNAs later, and have important theoretical significance and potential practical value.

Description

Technical field: [0001] The invention belongs to the fields of biotechnology and medicine, and relates to a method for detecting gout serum miRNAs biomarkers and their expression levels. Background technique: [0002] Gout is a long-term purine metabolism disorder or uric acid excretion disorder related to heredity, which leads to continuous increase of blood uric acid and deposition of urate crystals, and a group of clinical syndromes that cause tissue damage. Gouty arthritis is arthritis caused by the deposition of uric acid crystals formed in chronic hyperuricemia in the joint cavity. It is the most important disease of gout. With the change of people's lifestyle, the incidence of gout in our country is increasing, especially in the south and coastal economically developed areas, so it has been paid more and more attention by the medical community. The irreversible joint destruction and dysfunction caused by gout have brought great harm to people's health. Gout can not ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6883C12Q2600/178
Inventor 王颜刚赵世华侯旭
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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