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Early embryo diapause villus tissue miRNAs biomarkers and detection method of expression quantity thereof

A technology of biomarkers and detection methods is applied in the field of detection of miRNAs biomarkers and their expression levels in early embryonic aborted villous tissue, which can solve the problems of affecting the physical and mental health of women of childbearing age, unclear etiology, and limited treatment methods.

Active Publication Date: 2018-10-12
THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV +1
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Problems solved by technology

So far, the cause of early embryo cessation is unknown, and treatment options are limited, seriously affecting the physical and mental health of women of childbearing age
There are no published reports on miRNAs in early embryonic villus tissue

Method used

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  • Early embryo diapause villus tissue miRNAs biomarkers and detection method of expression quantity thereof
  • Early embryo diapause villus tissue miRNAs biomarkers and detection method of expression quantity thereof
  • Early embryo diapause villus tissue miRNAs biomarkers and detection method of expression quantity thereof

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Embodiment Construction

[0032] The present invention will be described in detail below by means of the examples in conjunction with the accompanying drawings, and the conditions and methods not indicated in the examples are all carried out according to conventional conditions.

[0033] In this example, miRNAs biomarkers of early embryo cessation villus tissue are used to detect early embryo cessation, and the specific process is as follows:

[0034] 1. Use microRNAs high-throughput sequencing to screen candidate early embryo stop-related miRNAs:

[0035]The villi tissues of 3 normal early pregnancy healthy controls and 3 early embryo stop-fertility patients were collected, and the total RNA was extracted by Trizol method, and the quality inspection of the obtained total RNA was carried out to ensure that the concentration and integrity of the total RNA met the sequencing requirements . Take 1.5 μg of total RNA from each sample, use the small RNA enrichment kit to enrich the 18-32nt fragment, use the...

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Abstract

The invention discloses early embryo diapause villus tissue miRNAs biomarkers related to early embryo diapause degree,which includes hsa-miR-136-5p,hsa-miR-1301-3p,hsa-miR-135b-5p,hsa-141-3p,hsa-miR-19b-3p and hsa-iR-486-5p. Accordingly,the inventor also designs the corresponding reverse transcription or real-time fluorescent PCR primers aiming at the miRNAs biomarkers. The biomarkers can be usedas biomarkers of a fluff sample for detecting early embryo diapause,or used for preparing a diagnostic reagent or a biochip and the like,and has important potential value and application prospect on the diagnosis and treatment of early embryo diapause,can provide a theoretical basis for the later diagnosis of early embryo diapause from the molecular level and research of tissue and serum miRNAs,and has important theoretical significance and potential practical value.

Description

technical field [0001] The invention belongs to the technical field of biomarkers in biomedicine, and in particular relates to a method for detecting miRNAs biomarkers and expression levels of early embryo cessation villus tissue. Background technique [0002] Early embryo arrest (Early Embryonic Arrest, EEA) is one of the main causes of female infertility. So far, the cause of early embryo cessation is still unclear, and treatment options are limited, seriously affecting the physical and mental health of women of childbearing age. [0003] MicroRNAs (miRNAs) are an important regulatory molecule in modern biomedicine. Through the regulation of gene transcription and expression, they regulate various functions of all cells, tissues and organs in the human body, and play a very important role in the occurrence and prevention of human diseases. important role. A large number of studies have found that miRNAs can widely and stably exist in extracellular fluid, including serum,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/113C12N15/11
CPCC12Q1/6851C12Q1/6883C12Q2600/158C12Q2600/136C12Q2600/178C12Q2531/113C12Q2521/107C12Q2563/107C12Q2525/207
Inventor 庞丽红杨文梅邓翎洁玉宁韦懿芸植枝福聂秋苗唐巧燕
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGXI MEDICAL UNIV
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