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A kind of method of biological control anti-rhodiola root rot and growth promotion

A rhodiola root and rhodiola technology, applied in pest control, botany equipment and methods, chemicals for biological control, etc., can solve the problem of low stability, low stress resistance of non-spore bacteria, Poor resistance and other problems, to achieve the effect of simple treatment, good economic benefits, and no waste water discharge

Inactive Publication Date: 2017-01-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, non-spore bacteria have low stress resistance and poor resistance to dry high temperature, so the stability is not high when made into liquid or adsorbent

Method used

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  • A kind of method of biological control anti-rhodiola root rot and growth promotion
  • A kind of method of biological control anti-rhodiola root rot and growth promotion
  • A kind of method of biological control anti-rhodiola root rot and growth promotion

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Experimental program
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Effect test

Embodiment 1

[0026] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.20×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated 2%, shake culture at 37°C for 24 hours, and the density of the bacterial solution is 2.21×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0027] 2) Cut a total of 100kg of healthy rhodiola branches into small sections for natural composting, spray 2L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, and continue composting until the internal temperature of the compost exceeds 43°C When the composting is over, it is stirred and cooled, and post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing Bacillus subtilis, which...

Embodiment 2

[0037] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.65×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated The amount is 3%, cultured with shaking at 37°C for 24 hours, and the density of the bacterial solution is 2.98×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0038] 2) Cut a total of 100 kg of healthy rhodiola branches into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, and continue composting until the internal temperature of the compost exceeds 43°C When the composting is over, it is stirred and cooled, and post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing B...

Embodiment 3

[0044] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.76×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated The amount is 3%, cultured with shaking at 37°C for 24 hours, and the density of the bacterial solution is 2.84×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0045] 2) Cut a total of 100 kg of healthy rhodiola branches into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, and continue composting until the internal temperature of the compost exceeds 43°C When the composting is over, it is stirred and cooled, and post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing B...

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Abstract

The invention discloses a method for root rot resistance and growth promotion of rhodiola rosea through biological prevention and control. The method includes the following steps that a high-density bacterium suspension with the density of 10<9> cfu / mL-10<10> cfu / mL is prepared through bacillus subtilis; healthy branches of rhodiola rosea are cut into small sections for composting, the high-density bacterium suspension of bacillus subtilis is sprayed in the composting process, biological fertilizer capable of effectively restraining and killing rhodiola rosea root rot disease bacteria is obtained after fermentation, and the biological fertilizer is applied to root rot resistance and growth promotion of rhodiola rosea through biological prevention and control. According to the method, bacillus subtilis producing beta dextranase is plant probiotics playing a role in disease resistance and growth promotion of rhodiola rosea, bacillus subtilis is added to compost, the restraining and killing effects on rhodiola rosea root rot disease bacteria are remarkably improved, and the fresh root weight of rhodiola rosea is remarkably increased. Treatment is simple and convenient in the composting process, no wastewater is discharged, investment is small, energy consumption is low, operation is easy, industrial large-scale production is facilitated, and good economic benefits and wide application prospects are achieved.

Description

technical field [0001] The invention relates to the field of preparation of biological control fertilizers, in particular to a method for biological control of rhodiola root rot and growth promotion. Background technique [0002] Rhodiola rosea (scientific name: Rhodiola rosea L.) is a perennial herb with a height of 10-20 cm. The roots are thick, conical, fleshy, brownish-yellow, with many fibrous roots at the root neck, short, thick, cylindrical rhizomes, covered with many scale-like leaves arranged in tiles, growing in the alpine and pollution-free zone at an altitude of 1800-2500 meters , its growth environment is harsh, so it has strong vitality and special adaptability. Rhodiola rosea can be used as medicine, it can invigorate qi and clear the lungs, improve intelligence and nourish the heart. It is a traditional Chinese medicine with a wide range of functions, and it also has cosmetic effects. It can be used as skin care products and can also be eaten. Rhodiola root...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G13/00A01N63/00A01P3/00A01P21/00
CPCA01G13/00A01G22/00A01N63/00
Inventor 吴渊胡慧雯徐薇薇阮晖梁莹许丽珍吴孔华莫建平史金昕波缪丹俞雨吴盛彬徐腾洋
Owner ZHEJIANG UNIV
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