Natural killer cell culture medium and natural killer cell amplification culture method

Abstract

The invention relates to the cell culture technical field, and particularly relates to a natural killer cell culture medium and a natural killer cell amplification culture method. The invention provides the culture medium used for amplification culture of natural killer cells and containing a serum-free culture medium, human plasma, IL-2, IL-21, IL-15 and OKT-3. The culture medium provided by the invention is used for amplification culture of the natural killer cells, can avoid risks caused by exogenous serum, has high amplification efficiency and allows the obtained natural killer cells to have high purity. With adopting the method for amplification of the natural killer cells, amplification of the natural killer cells can be maintained at a logarithmic phase for a longer period of time. Moreover, the cultured natural killer cells have good killing activity on tumor cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a medium for natural killer cells and a method for expanding and culturing natural killer cells. Background technique [0002] Natural killer cells (NK), also known as NK cells, are considered to be the body’s first line of defense against infection and tumors. Associated with, and in some cases involved in, the development of hypersensitivity reactions and autoimmune diseases. NK cells are rich in cytoplasm and contain larger azurophilic granules, and the content of granules is positively correlated with the killing activity of NK cells. The killing effect of NK cells occurs early after acting on the target cells, and the killing effect can be seen in 1 hour in vitro and 4 hours in vivo. The target cells of NK cells mainly include certain tumor cells (including some cell lines), virus-infected cells, some self-tissue cells (such as blood cells), parasites, etc. Therefore,...

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Problems solved by technology

[0003] At present, most NK cell culture systems use fetal bovine serum plus basal medium for culture, but the use of fetal bovine serum for culture will have potential risks brought by heterologous serum

For this reason, it has also been reported that NK cells were cultured in serum-free medium supplemented with cytokines IL-2 or IL-15 to avoid the risk of heterologous serum, but the effect of expanding NK was not obvious and the purity was relatively low; Unable to reach clinically required cell numbers

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