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Red back leaf root extract, preparation method and application in preparation of medicine for treating hepatitis B

A technology for root and extract of red back leaves, which is applied in the directions of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problems of long maintenance time and the like, and achieve the effect of simple and reasonable extraction process.

Active Publication Date: 2018-12-04
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its effect is weaker than that of lamivudine, but it lasts for a long time and is safe to take

Method used

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  • Red back leaf root extract, preparation method and application in preparation of medicine for treating hepatitis B

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preparation example Construction

[0022] The present invention is a kind of preparation method of red back leaf root extract, and it comprises the following steps:

[0023] (1) The medicinal material of the root of the red back leaf is extracted with 6-12 times the amount of ethanol for 1-2 hours, extracted 1-3 times, and the ethanol extracts are combined, filtered and concentrated under reduced pressure. Preferably, in step (1), the ethanol concentration is 60%-90%, and more preferably, the ethanol concentration is 70%-80%. The red-backed leaf root is the root of Alchornea trewioides (Benth.) Muell.–Arg., a plant of the family Euphorbiaceae.

[0024] (2) Centrifuge the concentrated solution obtained in step (1), and concentrate the supernatant under reduced pressure for subsequent use.

[0025] (3) The concentrated solution obtained in step (2) is placed on a medium-polar or non-polar macroporous adsorption resin column at a flow rate of 1.3-1.7 column volume / hour.

[0026] (4) Elution with deionized water ...

Embodiment 1

[0031] The medicinal material of the red back leaf root was extracted twice with ethanol, the concentration of ethanol was 75%, the amount of alcohol added was 10 times the weight of the medicinal material, and the extraction time was 90 min. The extracts were combined, filtered and concentrated under reduced pressure, the concentrate was centrifuged, and the supernatant was concentrated under reduced pressure for use.

[0032] Take the medium-polar macroporous adsorption resin, pack it into the column at a ratio of 1:2 by weight to the crude drug, and then pass the above-mentioned spare concentrate through the macroporous resin column at a flow rate of 1.5 column volumes / hour. Elute with deionized water, followed by 30-50% ethanol at a flow rate of 2.5 column volumes / hour. The ethanol eluate was collected, dried under reduced pressure, and pulverized to obtain the root extract of the red back leaf. The yield was 0.38%.

Embodiment 2

[0034] The medicinal material of the root of the red back leaf was extracted three times with ethanol, the concentration of ethanol was 90%, the amount of alcohol added was 12 times the weight of the medicinal material, and the extraction time was 120 min. The extracts were combined, filtered and concentrated under reduced pressure, the concentrate was centrifuged, and the supernatant was concentrated under reduced pressure for use.

[0035] Take the medium-polar macroporous adsorption resin, pack it into the column at a ratio of 1:2 by weight to the crude drug, and then pass the above-mentioned spare concentrate through the macroporous resin column at a flow rate of 1.5 column volumes / hour. Elute with deionized water, followed by 30-60% ethanol at a flow rate of 2.5 column volumes / hour. The ethanol eluate was collected, dried under reduced pressure, and pulverized to obtain the root extract of the red back leaf. The yield was 0.29%.

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Abstract

The invention discloses a redback christmashush extract, and a preparation method and application thereof in preparation of drugs for treating hepatitis B. The preparation method comprises the steps of carrying out alcohol extraction, centrifugal concentration, macroporous adsorption resin column treatment, ethanol elution and reduced pressure drying, so as to obtain an effective part extract of redback christmashush. The preparation method has the beneficial effects that an extraction process is simple, convenient and reasonable; by applying the obtained effective part extract of redback christmashush in an anti-duck hepatitis B virus experiment, serum DHBV DNA can be remarkably inhibited on the seventh day and the fourteenth day (T7 and T14) by virtue of three doses of effective part extracts of redback christmashush (P is less than 0.01), and DHBV DNA rises again on fifth day (P5) after the drug delivery is stopped and is still remarkably lower than that before the drug delivery, so that the redback christmashush extract has a relatively ideal anti-hepatitis B virus effect, is a candidate drug with great promise and can be applied to the preparation of anti-hepatitis B drugs.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and particularly relates to an extract of effective parts of the root of the red back leaf, and also relates to a preparation method and application of the extract. Background technique [0002] Hepatitis B is a disease caused by hepatitis B virus (HBV) that seriously endangers human health. There are about 120 million hepatitis B patients and HBV carriers in China. The study found that 60% to 70% of "asymptomatic hepatitis B virus carriers" were reported as chronic persistent hepatitis or chronic active hepatitis in liver biopsy pathology reports. Long-term hepatitis B virus infection can develop into liver cirrhosis and even liver cancer. Hepatitis B virus has caused great harm to the health and life safety of Chinese residents. Currently, the drugs widely used in clinical treatment of HBV infection are interferon (IFN) and nucleoside drugs. IFN is an anti-HBV drug with a relativ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/47A61P1/16A61P31/20
Inventor 吕志平刘强
Owner SOUTHERN MEDICAL UNIVERSITY
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