A preparation method and application of petroleum-degrading bacteria immobilized embedding particles

A petroleum-degrading bacteria and petroleum-degrading technology are applied in the field of preparation of immobilized and embedded particles of petroleum-degrading bacteria, and can solve the problems of not being able to guarantee the adsorption capacity of a carrier to the bacteria, affecting the activity of the bacteria, etc., so as to improve the microbial degradation ability and improve the Good permeability and mass transfer performance

Active Publication Date: 2018-01-16
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method directly embeds the bacteria, which affects the activity of the bacteria, and cannot guarantee the amount of adsorption of the carrier to the bacteria. The prepared fly ash immobilized particles have the highest removal rate of 54.1% for soil PYR and BaP. % and 45.8%, the removal rates of other immobilized particles to PYR and BaP were lower than 50% and 40%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of auxiliary carrier

[0023] Water hyacinth and wheat straw were dried and crushed, passed through a 80-mesh sieve, and then mixed with walnut shell activated carbon, wherein water hyacinth was 46.5wt%, wheat straw was 18.5wt%, and walnut shell activated carbon was 35wt%. The mixture was soaked in distilled water for 24 hours, adjusted to pH 7.0, the floating impurities and supernatant in the upper layer were filtered off, the filtered mixture was dried at 50°C for 24 hours, sterilized by high-pressure steam at 121°C for 20 minutes, and used as an auxiliary carrier.

[0024]The total carbon content of the water hyacinth in the auxiliary carrier is 250g / kg, the total nitrogen content is 43.8g / kg, and the total phosphorus content is 10.2g / kg. The total carbon content of wheat straw is 32.6g / kg, the total nitrogen content is 40.8 g / kg, and the total phosphorus content is 0.76g / kg. Walnut shell activated carbon is powder activated carbon with a specific sur...

Embodiment 2

[0035] One of the differences from Example 1 is that in the pretreatment process of the auxiliary carrier, the ratio of each material is: water hyacinth 43wt%, wheat straw 17wt%, walnut shell activated carbon 40wt%. The second difference is: when preparing the oil-degrading bacteria seed liquid, ensure that the number of oil-degrading bacteria in the seed liquid reaches 3×10 10 individual / mL. The third difference is that when preparing the immobilized embedding particles of petroleum-degrading bacteria, the mass of polyvinyl alcohol is 10wt%, sodium alginate 1.5wt%, CaCl 2 The concentration of the solution is 3wt%, in 10wt% Na 2 SO 4 The reaction time in solution was 2h.

[0036] Other conditions are the same, under the condition of repair time of 40 days, the removal rate of petroleum pollutants can reach 64.7%, and the removal rate of saturated hydrocarbons can reach 73%.

Embodiment 3

[0038] One of the differences from Example 1 is that in the pretreatment process of the auxiliary carrier, the ratio of each material is: water hyacinth 35.8wt%, wheat straw 14.2wt%, walnut shell activated carbon 50wt%. The second difference is: when preparing the oil-degrading bacteria seed liquid, ensure that the number of oil-degrading bacteria in the seed liquid reaches 5×10 10 individual / mL. The third difference is that when the petroleum-degrading bacteria immobilized embedding particles were prepared, the auxiliary carrier was added at 3wt%, and cultured at 120r / min and 30°C for 24h. The quality of adding PVA is 8wt%, sodium alginate 1.2wt%, CaCl 2 The concentration of the solution is 5wt%, at 10wt% Na 2 SO 4 The reaction time in solution was 2h. The fourth difference is that the oily soil contains 50g / kg of oil, and the amount of embedded particles immobilized by petroleum-degrading bacteria is 1kg / m 2 .

[0039] Other conditions are the same, under the repair ti...

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Abstract

The invention relates to a preparation method of immobilized embedding particles of petroleum-degrading bacteria, comprising: (1) mixing 35.8wt%-46.5wt% of water hyacinth, 14.2wt%-18.5wt% of wheat straw and 35wt%-50wt% walnut shell activated carbon mixed as auxiliary carrier; (2) take 3.0wt%~7.0wt% auxiliary carrier, insert 10wt%~20wt% oil-degrading bacteria seed solution for adsorption; (3) add 8.0wt%~ Dissolve 10wt% polyvinyl alcohol in water at 80~100℃, add 1.2wt%~1.5wt% sodium alginate and mix well, add the rest of water after cooling; (4) Mix (2) and (3) to form a package Embedded in the system, dropped into CaCl2 solution to cross-link into balls, and then transferred to Na2SO4 solution for immobilization to obtain immobilized embedding particles. The immobilized embedding particles of petroleum-degrading bacteria prepared by the invention are applied to the in-situ restoration of petroleum-contaminated soil, and have a good restoration effect on petroleum-contaminated soil.

Description

technical field [0001] The invention relates to the field of microorganism immobilization, in particular to a preparation method and application of petroleum-degrading bacteria-immobilized embedding particles for repairing petroleum-contaminated soil. Background technique [0002] Petroleum-contaminated soil is one of the important pollutions of petroleum and petrochemical enterprises, and the petroleum pollutants in it have a high poisonous effect on human body and environment. Soil pollution has attracted more and more attention, and it is becoming more and more important to ensure the efficiency of soil remediation and restore healthy production. [0003] The treatment methods of petroleum-contaminated soil at home and abroad mainly include incineration, landfill, stacking and bioremediation. However, the biological method is the most economical and thorough method, and it is the most effective means to achieve soil ecological restoration. In order to solve the shortcom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14B09C1/10
Inventor 秦丽姣张胜中邓德刚周彤
Owner CHINA PETROLEUM & CHEM CORP
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