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Bee pollen extract as well as preparation and application thereof

A technology of bee pollen and extract, which is applied in the fields of food, pharmacy, and biochemical industry. It can solve the problems that the full effect of bee pollen cannot be reflected, the active ingredient of the extract is single, and no bee pollen has been seen. It has good application prospects and controllable residues. , the effect of reducing the effective dose

Active Publication Date: 2015-04-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, bee pollen has the problems of high dosage required for direct use of the original drug, and the single active ingredient of the extract cannot reflect the full effect of bee pollen. So far, there is no preparation method for retaining the full effect of bee pollen

Method used

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  • Bee pollen extract as well as preparation and application thereof
  • Bee pollen extract as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] One, the preparation method of bee pollen extract

[0022] Rapeseed pollen was used as raw material to prepare bee pollen extract according to the following process:

[0023] Take 1000 grams of dried bee pollen, add 5L of ethanol with a volume concentration of 70%, and extract at 20°C for 10 hours. After the extraction is completed, filter and separate the extract from the solids for processing:

[0024] (1) The treatment method of the extract: the extract is concentrated and dried in vacuum, the concentrated sample is dissolved in ethanol with a volume concentration of 70%, and then put on a macroporous resin column, first rinsed with deionized water of 5 times the column volume, and then with a volume concentration of The column was washed with 70% ethanol and fractions were collected, and the fraction washed with 70% ethanol was concentrated and dried in vacuo to obtain fraction 1.

[0025] (2) Solid matter treatment method: add water to the solid matter according t...

Embodiment 2

[0095] Using tea bee pollen as raw material, the bee pollen extract is prepared according to the following process:

[0096] Take 1,000 grams of dried tea bee pollen, add 50 L of ethyl acetate, and extract at 50°C for 2 hours. After the extraction is completed, filter and separate the extract from the solids for processing:

[0097] (1) The treatment method of the extract: the extract is concentrated and dried in vacuum, the concentrated sample is dissolved in 70% ethanol and then put on a macroporous resin column, first rinsed with deionized water of 5 times the column volume, and then washed with 70% ethanol The column was collected and fractions were collected, and the fraction washed with 70% ethanol was concentrated and dried in vacuo to obtain fraction 1.

[0098] (2) Solid matter treatment method: solid matter is added 0.01M Tris-HCl buffer solution according to the ratio of raw material: solution = 1kg: 2L, the pH of the mixed solution is adjusted to be in the range of...

Embodiment 3

[0102] Using corn bee pollen as raw material, the corn bee pollen extract is prepared according to the following process:

[0103] Take 1,000 grams of dried corn bee pollen, add 20 L of n-hexane, and extract at 30°C for 5 hours. After the extraction, filter and separate the extract from the solids for processing:

[0104] (1) The treatment method of the extract: the extract is concentrated and dried in vacuum, the concentrated sample is dissolved in 70% ethanol and then put on a macroporous resin column, first rinsed with 10 times the column volume of deionized water, and then washed with 50% ethanol The column was collected and the fractions were collected. The fractions washed with 50% ethanol were combined and then concentrated and dried in vacuo to obtain Fraction 1.

[0105] (2) Solid matter treatment method: solid matter is added with 0.05M phosphate buffer solution according to the ratio of raw material: solution = 1kg:5L, the pH of the mixed solution is adjusted to 7, ...

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Abstract

The invention discloses a preparation method for a bee pollen extract. The preparation method specifically comprises the following steps: extracting a flavonoids compound of bee pollen, preparing biological active peptide in the bee pollen by using extraction residues, and preparing the bee pollen extract by mixing the flavonoids compound and the biological active peptide. The prepared bee pollen extract has the activity of both the active peptide and the flavonoids compound.

Description

technical field [0001] The present invention relates to a preparation method of bee pollen extract, specifically extracting flavonoids from bee pollen first, then using the extraction residue to prepare bioactive peptides in bee pollen, and then mixing flavonoids and active peptides to prepare bee pollen Extract, the prepared bee pollen extract has both the activity of active peptides and flavonoids. The invention belongs to the technical fields of biochemical industry, pharmacy, food and the like. Background technique [0002] Bee pollen is the pollen grains collected by honey from flowering plants, which are formed by adding honey's own glandular secretions, saliva and nectar during the collection process. Bee pollen is one of the main sources of nutrition for the growth and reproduction of bee colonies, and has high nutritional value. Bee pollen contains protein, carbohydrates, flavonoids, trace elements, enzymes and other nutrients, so bee pollen has always been known ...

Claims

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Application Information

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IPC IPC(8): A61K36/31A23L1/29A61K8/97A61P9/00A61P37/04A61P3/10A61P39/06A61P13/08A61K133/00A23L33/00
Inventor 邹汉法靳艳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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