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Application of pyrazinamide in the preparation of drugs for inhibiting tumor cell metastasis and spread

A technology of tumor cells and pyrazinamide, applied in the application field of medicine, can solve the problem of undiscovered pharmacological effects of pyrazinamide, and achieve the effect of shortening the research and development cycle and reducing the number of deaths

Inactive Publication Date: 2016-08-17
江苏澳格姆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Other pharmacological effects of pyrazinamide have not been found in the existing published literature

Method used

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  • Application of pyrazinamide in the preparation of drugs for inhibiting tumor cell metastasis and spread
  • Application of pyrazinamide in the preparation of drugs for inhibiting tumor cell metastasis and spread
  • Application of pyrazinamide in the preparation of drugs for inhibiting tumor cell metastasis and spread

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: The application of pyrazinamide in the preparation of drugs for inhibiting the metastasis and spread of colon cancer cells. The specific experimental plan is as follows:

[0024] 1. Experimental materials

[0025] Colon cancer cells (HT29) were used in the experimental stage of the present invention, and the drug used in the positive control was nocodazole (experimental concentration: 10uM). The negative control group received no treatment.

[0026] Wherein experiment adopts pyrazinamide compound C 5 h 5 N 3 O, CAS NO. is 98-96-4, the concentration is 10uM, 5uM, 1uM, 0.5uM respectively.

[0027] 2. Experimental method

[0028] For colon cancer cells cultured for 24 hours (cell density: 10 5 ) for drug stimulation for 2 hours:

[0029] (1) Centrifuge the colon cancer cells in a better state in the culture dish, and add an appropriate amount of culture medium to make a cell suspension;

[0030] (2) Get the cell suspension count, according to 10 5 The ...

Embodiment 2

[0038] Example 2: Transwell cell migration assay was used to determine the effect of pyrazinamide on the migration ability of colon cancer cells.

[0039] Will 1×10 5 The HT29 cells were inoculated in Transwell chambers with a 12.0 μm membrane at the bottom, and continued to be cultured. After the cells adhered to the wall, DMEM complete medium with different concentrations of pyrazinamide was added to the upper chamber. To maintain the osmotic pressure, 0.05%-0.2% BSA was added. The lower chamber was added with DMEM complete medium containing 15% FBS and continued to culture for 24 hours. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 2% PFA at 37°C for 10 minutes and then stained with Giemsa. Ten visual fields were randomly selected under a high-power microscope to count the migrated cells.

[0040] Cell migration rate = [the average numb...

Embodiment 3

[0044] Example 3: The damage repair experiment detects the effect of pyrazinamide on the migration ability of tumor cells.

[0045] Take HT29 cells in the logarithmic growth phase, and add 1×10 cells to each well of a 24-well plate 5 After the cells grow into a single layer, draw a "one"-shaped scratch, rinse with PBS, add different concentrations of pyrazinamide to the control group and the experimental group, and take pictures. After 22 hours of culture, the same part is photographed again to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / (0h average value of the blank control group-22h average value of the blank control group)]×100%.

[0046] Pyrazinamide inhibited the migration of LTEP and A549 cells at different concentrations (0.5-10 μg / ml) for 22 hours. The results showed that pyrazinamide had a dose-dependent inhibitory effect on t...

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Abstract

The invention discloses an application of pyrazinamide in preparation of medicines for inhibiting tumor cell metastasis and spread. The metastasis capability of tumor cells is inhibited by utilizing the medicines prepared from the pyrazinamide, the pyrazinamide refers to a low-toxicity medicine, a great blocking effect can be achieved in the tumor spreading process, meanwhile, treatment is performed by adopting other related tumor drugs, and the death amount of normal cells can be greatly reduced; and moreover, most of the existing tumor drugs are cytotoxic drugs, and lots of normal cells are killed when the tumor cells are killed.

Description

technical field [0001] The invention relates to a new application of pyrazinamide in the field of pharmacy, in particular to the application of pyrazinamide in the preparation of drugs for inhibiting tumor cell metastasis and spread. Background technique [0002] Pyrazinamide is similar to nicotinamide, which can play a bactericidal effect when entering cells. Under the condition of low pH, the antibacterial activity is strong. This product is the main component of the second-line anti-tuberculosis drug, and has no cross-resistance with other anti-tuberculosis drugs. It is mainly used for cases where the first-line drug treatment is ineffective. Single use of this product is prone to drug resistance and needs to be used in combination with other anti-tuberculosis drugs. [0003] The mechanism of action may be that PZA penetrates into the macrophages containing Mycobacterium tuberculosis and converts it into pyrazinic acid to play an antibacterial effect. This product is a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4965A61P35/04
CPCA61K31/4965
Inventor 滕文臣
Owner 江苏澳格姆生物科技有限公司
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