A method for the separation and determination of coniferyl alcohol and sinapyl alcohol by high performance liquid chromatography

Abstract

The invention discloses a method for separating and detecting the contents of coniferyl alcohol and sinapyl alcohol by using high performance liquid chromatography. It breaks the conventional method of determining coniferyl alcohol by high-phase gas chromatography, and this method can detect coniferyl alcohol and sinapyl alcohol at the same time. In addition, the method of measuring under the same conditions as acid substances can be realized, so that the experimental data can explain the problem better. The method uses a C18 column, the mobile phase is water and methanol, the volume flow rate is 0.4-0.7mL / min, the column temperature is 25-30°C, the analysis time is 70min, the detection wavelength is 275nm, and the injection volume is 20uL. Detection and separation of coniferyl alcohol and sinapyl alcohol, and better peak shape, can be used to separate and measure coniferyl alcohol and sinapyl alcohol in samples.

Description

technical field [0001] The invention belongs to the field of substance separation and measurement, and in particular relates to a method for separating and measuring the contents of coniferyl alcohol and sinapyl alcohol by high performance liquid chromatography. Background technique [0002] Both coniferyl alcohol and sinapyl alcohol are less studied chemicals, the structural formula of coniferyl alcohol is given in figure 1 , the structural formula of sinapyl alcohol is shown in figure 2 , many of their physical and chemical properties are unknown, more in-depth research is needed for coniferyl alcohol and sinapyl alcohol, and they are key intermediate metabolites in the pathway of pear secondary metabolism to form lignin, coniferyl alcohol and sinapyl alcohol are G - Precursor substances of lignin and S-lignin. Studying coniferyl alcohol and sinapyl alcohol is beneficial to better study the metabolic pathway of pear. The taste of pears is related to stone cells, and st...

AI Technical Summary

Problems solved by technology

[0004] In addition, the structures of coniferyl alcohol and sinapyl alcohol are similar. Compared with coniferyl alcohol, sinapyl alcohol has only one more methoxy group. Through the wavelength scanning of the ultraviolet spectrophotometer, we can see that the peak shapes of the two are consistent, and at the same detection wavelength In this case, the absorbance of the two is the same, and the HPLC detection light is ultraviolet light, so it is still difficult to separate and detect coniferyl alcohol and sinapyl alcohol by HPLC, which has never been done before.

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