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Microsatellite marker method for identifying wild odontobutis potamophila population of minjiang river basin

A technology of microsatellite marker and sand pond snakehead, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of improving identification efficiency and accuracy, and simple and quick operation.

Inactive Publication Date: 2015-04-22
NANJING NORMAL UNIVERSITY
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Problems solved by technology

At present, there is no quick and easy method for the identification of wild populations of Channa sinensis using microsatellite molecular marker technology

Method used

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  • Microsatellite marker method for identifying wild odontobutis potamophila population of minjiang river basin
  • Microsatellite marker method for identifying wild odontobutis potamophila population of minjiang river basin
  • Microsatellite marker method for identifying wild odontobutis potamophila population of minjiang river basin

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Embodiment Construction

[0025] An example of the microsatellite marker method for identifying the population of wild river snakeheads in the Minjiang River Basin of the present invention is as follows, and the specific parameters are required for illustrating the technical solution of the present invention, and should not be interpreted as limiting the protection scope of the present invention.

[0026] A microsatellite marker method for identifying wild river channa populations in the Minjiang River Basin, comprising the following steps:

[0027] 1) DNA extraction:

[0028] Five representative populations of the distribution of the wild river channa were selected (the wild population in Dangtu area, the wild population in Sheyang area, the wild population in Yuyao in the Yongjiang River basin, the wild population in the Minjiang River basin, and the wild population in Dongxishan in the Taihu Lake basin) (hechuan Channa is mainly distributed in my country's Yangtze River, Qiantang River, and Minjiang ...

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Abstract

The invention discloses a microsatellite marker method for identifying wild odontobutis potamophila population of a minjiang river basin. The method comprises the following steps: odontobutis potamophila genome DNA extraction, DNA segment amplification, PCR product electrophoresis and microsatellite site strip analysis. If a specific DNA strip is amplified from a detection sample only at the 259bp position close to a standard map, the odontobutis potamophila is identified to be the odontobutis potamophila population of the minjiang river basin; and if one or two specific strips are amplified from the detection sample only at the 263bp and 265bp positions close to the standard map, odontobutis potamophila is identified to be wild odontobutis potamophila populations in other river basins. The method is simple, fast and accurate to operate, and can be applied to identification of the odontobutis potamophila population of the minjiang river basin, thus the method is of important significance on genetic resources protection of the odontobutis potamophila population of the river basin.

Description

technical field [0001] The invention belongs to the technical field of molecular identification of wild populations in aquaculture, and in particular relates to a microsatellite marking method for identifying wild river snakehead populations in the Minjiang River Basin. technical background [0002] Odontobutis potamophila belongs to the order Perciformes, family Odontobutidae, and the genus Odontobutis. In my country, it is widely distributed in the middle and lower reaches of the Yangtze River, Minjiang River, Qiantang River and other water systems, and belongs to China's endemic species. The fish is a freshwater benthic small carnivorous fish with tender meat, delicious taste and rich nutrition, and is deeply loved by the people. In recent years, with the sharp decline of wild resources and the continuous growth of market demand, the river snakehead has developed into a very promising new species of aquaculture. At present, the polyculture and nesting of sand pond snake...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2525/151C12Q2531/113C12Q2565/125
Inventor 尹绍武祝斐张国松
Owner NANJING NORMAL UNIVERSITY
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