A rapid and sensitive method for the detection of Plasmodium malariae

A technology for sensitive detection of Plasmodium, applied in the field of in vitro diagnostic reagents, can solve problems such as complex operation, and achieve the effect of improving detection ability, reducing time and complexity

Active Publication Date: 2017-12-26
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The current gene chip detection for malaria parasite detection has been used in some researches, but the probes of these gene chips are mostly placed in a manual mode, which is complicated to operate and increases the chance of many false positives

Method used

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  • A rapid and sensitive method for the detection of Plasmodium malariae
  • A rapid and sensitive method for the detection of Plasmodium malariae
  • A rapid and sensitive method for the detection of Plasmodium malariae

Examples

Experimental program
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Effect test

Embodiment 1 3

[0037] Example 1 Specificity and sensitivity of detection of Plasmodium vivax

[0038] 1. Experimental steps

[0039] 1. Collection of blood samples

[0040] Take 1.0ml of the whole blood sample of the patient tested at the port.

[0041] 2. Preliminary identification of blood samples

[0042] The colloidal gold method was used to preliminarily determine whether the patient was infected with Plasmodium vivax.

[0043] 3. Extraction of genomic DNA

[0044] Use blood sample genomic DNA extraction kit (spin column type, Qiagen company) to extract the malaria vivax DNA. Take a 50uL whole blood sample and extract DNA according to the extraction steps in the kit instructions.

[0045] 4. Determination of DNA concentration

[0046] The DNA concentration of Plasmodium was quantified by real-time quantitative PCR using ABI's real-time fluorescence detector.

[0047] 5. Amplification of target genes

[0048] 5.1 Design of primers and probes

[0049] For the unique genes of Plasmodium vivax, design prim...

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Abstract

The invention relates to a rapid and sensitive plasmodium malariae detection method. The detection method integrates two powerful molecular biology techniques of a polymerase chain reaction PCR and a microarray, PCR hybridized probes are directly fixed on a hybridization cabin in the microarray and are in a same chip with a PCR reaction chamber; the detection method includes the steps of carrying out bacteria propagation treatment on a sample, extracting a DNA liquid, carrying out PCR amplification, hybridizing, cleaning, and reading and discriminating a result. The method can quickly and sensitively detect plasmodium malariae, can greatly improve the detection efficiency of front line inspection and quarantine personnel of import and export ports, not only can reduce the workload, but also furthest solves a positive detection missing problem possibly existing in a traditional detection method, so as to furthest prevent generation of plasmodium epidemic situation.

Description

Technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a rapid and sensitive method for detecting Plasmodium vivax. Background technique [0002] According to WHO statistics, malaria is prevalent and spread in 107 countries and regions around the world, 3.2 billion people are threatened by malaria, 300-500 million people suffer the disease every year, and the death toll reaches more than 1 million. Malaria is one of the five major parasites that our country focuses on. Malaria (malaria) is a serious parasitic disease caused by Plasmodium infection. There are 4 species of Plasmodium that infect humans: Plasmodium falciparum (Pf), Plasmodium vivax (Pv), Plasmodium malariae (Pm) and Plasmodium ovale (Plasmodium ovale, Po). ). Therefore, effective detection of malaria parasites is an indispensable means for medical diagnosis and malaria control. [0003] At present, microscopy is still the main method for monitoring pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/90
CPCC12Q1/6837C12Q1/6893C12Q2531/113C12Q2565/501Y02A50/30
Inventor 田桢干欧元祝陆晔邱德义张子龙岳巧云李深伟秦胜营李平
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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