Ecological restoration agent for fallow ginseng soil and preparation method of ecological restoration agent
An ecological restoration and soil technology, applied in the direction of biochemical equipment and methods, restoration of contaminated soil, and methods based on microorganisms, can solve problems such as continuous cropping obstacles in ginseng planting, and achieve the effect of promoting the growth of plant roots and degrading pesticides
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Embodiment 1
[0029] Step 1: Seed Production
[0030] Transfer Bacillus subtilis into the Erlenmeyer flask of nutrient broth liquid medium, culture at 38°C and 220rpm with shaking for 24 hours, take out after the medium is cloudy; transfer Saccharomyces cerevisiae into the Erlenmeyer flask of potato liquid medium , at 30°C, 220rpm shaking culture for 48 hours, take out after the medium is turbid; Nocardia was transferred into the Erlenmeyer flask of Gaussia No. 1 liquid medium, at 28°C, 220rpm shaking culture for 72 hours, to be cultured Take out after the base is turbid; transfer Lactobacillus plantarum into the Erlenmeyer flask of MRS liquid medium, culture at 37°C for 48 hours, take out after the medium is cloudy; transfer white rot bacteria into the Erlenmeyer flask of potato liquid medium culture at 28°C and 220rpm for 72 hours, and take it out after the medium is turbid; Rhodopseudomonas palustris was transferred into a conical flask of nutrient broth liquid medium, and cultured at 35...
Embodiment 2
[0035] Step 1: Corn Stalk Collection and Crushing
[0036] After the corn stalks are harvested, they are flattened in the field, dried, collected, and transported back to the production plant for temporary storage in the greenhouse, which is kept dry. The length of straw crushing is 3 mm, and the straw should be kept dry.
[0037] The second step: the collection and treatment of cow manure
[0038] Collect fresh cow manure and store it in the dung storage tank for later use. The water content of the manure is 85-95%;
[0039] The third step: preparation of soil ecological restoration agent in solid form
[0040] Mix the crushed corn stalks, cow dung, molasses and the above-mentioned compound soil ecological restoration bacteria solution in a weight ratio of 1:4:0.15:0.15 in a mixer evenly. Put it into an aluminum foil bag with an exhaust valve and seal it, and place it in a fermentation room at 28°C for complete anaerobic fermentation for two weeks to obtain the soil ecolog...
Embodiment 3
[0042] When the temperature is above 10°C, apply 1-2 tons of soil ecological restoration agent in solid form per mu of farmland, and turn it into the soil with a rotary tiller; sprinkle water to keep the soil humidity at 60-80%, and then evenly spray the soil ecological restoration in liquid form The dosage is determined according to the soil condition; after 60 days, the soil ecological restoration agent in liquid form is evenly sprayed again (the dosage is the same as the first time), and after 3 months, the soil restoration is basically completed.
[0043] Table 1 Changes of soil microorganisms before and after treatment:
[0044]
[0045] It can be seen from the above that the application of soil ecological restoration agent can significantly increase the number of microorganisms in the soil, and the application amount of soil ecological restoration agent is positively correlated with the number of microorganisms in the soil.
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