A rapid determination method for fumonisins in corn grains
A technology for rapid determination of fumonisins, applied in the field of analysis and detection, can solve the problem of difficulty in simultaneous detection of multiple mycotoxins, and achieve the effects of simple operation, accurate qualitative and quantitative accuracy.
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[0053] In order to better understand the present invention, the content of the present invention is further illustrated below in conjunction with the examples, but the content of the present invention is not limited to the following examples, and the examples should not be regarded as limiting the protection scope of the present invention.
[0054] 1 Instruments and samples
[0055] 1.1 Instruments:
[0056] Ultra-high performance liquid chromatography system (ACQUITYUltraPerformanceLCWaters Company) is equipped with mass spectrometer (G2-QTOF time-of-flight mass spectrometer Waters Company), N-EVAPTM112 nitrogen blowing instrument, ThermoHypersllGOLDC18 (1.9μm, 2.1×100mm) chromatographic column.
[0057] 1.2 Sample: corn
[0058] 2 Methods and results
[0059] 2.1 Extraction of fumonisins from corn kernels
[0060] Accurately weigh 2.0000g (accurate to 0.0001g) of the ground sample in a 50ml centrifuge tube. Add 20ml of extraction solution (acetonitrile + water + glacial ...
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Abstract
Description
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