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Corn kernel kumonisins rapid determination method

A rapid detection technology for fumonisins, which is applied in the field of analysis and detection, can solve problems such as the difficulty of simultaneous detection of multiple mycotoxins, and achieve the effects of simple operation, cost reduction, and accurate quantification

Inactive Publication Date: 2015-04-15
INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

High-performance liquid chromatography (HPLC) can simultaneously identify and quantify fumonisins, but derivatization is required for detection by HPLC, which makes it difficult to simultaneously detect multiple mycotoxins

Method used

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  • Corn kernel kumonisins rapid determination method
  • Corn kernel kumonisins rapid determination method
  • Corn kernel kumonisins rapid determination method

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Embodiment Construction

[0053] In order to better understand the present invention, the content of the present invention is further illustrated below in conjunction with the examples, but the content of the present invention is not limited to the following examples, and the examples should not be regarded as limiting the protection scope of the present invention.

[0054] 1 Instruments and samples

[0055] 1.1 Instruments:

[0056] Ultra-high performance liquid chromatography system (ACQUITY Ultra Performance LC Waters Company) equipped with mass spectrometer (G2-Q TOF time-of-flight mass spectrometer Waters Company), N-EVAPTM112 nitrogen blowing instrument, Thermo Hypersll GOLD C18 (1.9μm, 2.1×100mm) chromatography column.

[0057] 1.2 Sample: Corn

[0058] 2 Methods and Results

[0059] 2.1 Extraction of fumonisins from corn kernels

[0060] Accurately weigh 2.0000g (accurate to 0.0001g) of the ground sample in a 50ml centrifuge tube. Add 20ml of extraction solution (acetonitrile + water + gla...

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Abstract

The invention discloses a corn kernel kumonisins rapid determination method belonging to the technical fields of analysis and detection. The corn kernel kumonisins rapid determination method includes the following steps: (1) corn kernel kumonisins extraction; (2) extract purification; and (3) corn kernel kumonisins analysis and detection. Through the corn kernel kumonisins rapid determination method, corn kumonisins can be more rapidly and more fully extracted, the content of fumonisins can be more accurately measured, and the corn kernel kumonisins rapid determination method has great significance to improve the quality safety of corn.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a rapid determination method for fumonisins in corn grains. Background technique [0002] Fumonisin is a secondary metabolite produced by Fusarium sp. (mainly including Fusarium lamina, Fusarium pseudovertinosa, etc. Fumonisins in my country are mainly produced by Fusarium pseudovertinosa). The product is easily soluble in water. There are mainly 11 kinds of fumonisins discovered so far, including FA1, FA2, FB1, FB2, FB3, FC1, FC2, etc., among which FB1 is the most harmful. The study found that the fumonisins naturally occurring in corn are mainly FB1, FB2, and FB3. Fumonisins can harm crop growth at lower concentrations. The International Center for Cancer Research classifies fumonisins as probable human carcinogens. In 1990, Sydenham EW found that fumonisins can cause cancer and may be closely related to human esophageal cancer. Although fumonisins are less a...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 赵善仓张树秋邓立刚李增梅董艳婕陈业兵郭长英
Owner INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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