Frozen stock solution for storing mammary epithelial cells of milk goat and freeze-saving method

Abstract

The invention discloses a frozen stock solution for storing mammary epithelial cells of a milk goat. The frozen stock solution is prepared by mixing a basic frozen stock solution and insulin-transferrin-sodium selenate in a volume ratio of 100 to (1-2). The basic frozen stock solution is prepared by mixing 10% by volume percent of dimethyl sulfoxide, 30-50% by volume percent of a DMEM / F12 culture medium and 40-60% by volume percent of fetal calf serum. The freeze-saving frozen stock solution disclosed by the invention is few in step, simplified in process and low in cost, the resuscitated mammary epithelial cells are strong in anti-apoptosis performance, oxidization resistance and multiplication capacity, the resuscitation rate of the mammary epithelial cells can be increased, and the vitality of the resuscitated cells reaches up to above 95%, so that the cell growth quality is ensured, the proliferation cycle of cell resuscitation is shortened and the mammary epithelial cells of the milk goat can be saved for a long time.

Description

Technical field: [0001] The invention relates to the technical fields of tissue and cell engineering and cell biology, in particular to a cryopreservation solution and a cryopreservation method for preserving dairy goat mammary gland epithelial cells. Background technique [0002] Dairy goat mammary epithelial cells can not only be used as a model for studying milk protein gene expression and apoptosis, but also as a detection system for mammary gland-specific expression vectors. At present, although the method of in vitro isolation and culture of dairy goat mammary epithelial cells has been basically established, there is no recognized immortalized dairy goat mammary epithelial cell line. Dairy goat mammary epithelial cells are primary cultured cells, which need to be purified and subcultured to obtain functional mammary epithelial cells, which not only takes a long period of culture, but also causes a lot of waste of reagents and materials; As the number of cell cultures ...

AI Technical Summary

Problems solved by technology

In the above method, the protective component in the cell cryopreservation solution is single, there are many steps, and the interval is long. The negligence of each step will seriously affect the survival of the cells.

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